Hiroyuki Ijima &ndash; Research Output

2019

Analysis of sulfated glycosaminoglycans in ECM scaffolds for tissue engineering applications: Modified alcian blue method development and validation

Iimaa, T., Ikegami, Y., Bual, R., Shirakigawa, N. & Ijima, H., Jan 1 2019, In : Journal of Functional Biomaterials. 10, 2, 19.

Research output: Contribution to journal › Article

Open Access

A73025

Alcian Blue

Scaffolds (biology)

Tissue engineering

Heparin

A nano-sized gel-in-oil suspension for transcutaneous protein delivery

Hardiningtyas, S. D., Nagao, S., Yamamoto, E., Shirakigawa, N., Wakabayashi, R., Goto, M., Ijima, H. & Kamiya, N., Aug 15 2019, In : International Journal of Pharmaceutics. 567, 118495.

Research output: Contribution to journal › Article

Phycocyanin

Suspensions

Oils

Gels

Hydrogel

A novel evaluation system for whole-organ-engineered liver graft by ex vivo application to a highly reproducible hepatic failure rat model

Sakamoto, H., Shirakigawa, N., Bual, R. P., Fukuda, Y., Nakamura, S., Miyata, T., Yamao, T., Yamashita, Y. I., Baba, H. & Ijima, H., Sep 13 2019, In : Journal of Artificial Organs. 22, 3, p. 222-229 8 p.

Research output: Contribution to journal › Article

Liver Failure

Grafts

Liver

Rats

Transplants

Antibacterial-agent-immobilized gelatin hydrogel as a 3d scaffold for natural and bioengineered tissues

Iimaa, T., Hirayama, T., Shirakigawa, N., Imai, D., Yamao, T., Yamashita, Y. I., Baba, H. & Ijima, H., Jun 2019, In : Gels. 5, 2, 32.

Research output: Contribution to journal › Article

Open Access

Bactericides

Hydrogel

Gelatin

Hydrogels

Scaffolds

Development of heparin-conjugated nanofibers and a novel biological signal by immobilized growth factors for peripheral nerve regeneration

Ikegami, Y. & Ijima, H., Jan 1 2019, (Accepted/In press) In : Journal of Bioscience and Bioengineering.

Research output: Contribution to journal › Article

Nanofibers

Nerve Regeneration

Peripheral Nerves

Heparin

Intercellular Signaling Peptides and Proteins

Intact extracellular matrix component promotes maintenance of liver-specific functions and larger aggregates formation of primary rat hepatocytes

Bual, R. P. & Ijima, H., Dec 2019, In : Regenerative Therapy. 11, p. 258-268 11 p.

Research output: Contribution to journal › Article

Open Access

Liver

Extracellular Matrix

Rats

Hepatocytes

Maintenance

2 Citations (Scopus)

Liver-specific extracellular matrix hydrogel promotes liver-specific functions of hepatocytes in vitro and survival of transplanted hepatocytes in vivo

Ijima, H., Nakamura, S., Bual, R. P. & Yoshida, K., Sep 1 2019, In : Journal of Bioscience and Bioengineering. 128, 3, p. 365-372 8 p.

Research output: Contribution to journal › Article

Hydrogel

Hydrogels

Liver

Extracellular Matrix

Hepatocytes

2018

Current status and new challenges of the artificial liver

Mizumoto, H., Shirakigawa, N. & Ijima, H., Jun 27 2018, Biomedical Engineering Challenges: A Chemical Engineering Insight. Wiley-Blackwell, p. 27-54 28 p.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Decellularization of liver and organogenesis in rats

Shirakigawa, N. & Ijima, H., Jan 1 2018, Methods in Molecular Biology. Humana Press Inc., p. 271-281 11 p. (Methods in Molecular Biology; vol. 1577).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Organogenesis

Liver

Octoxynol

Blood Vessels

Hepatocytes

14 Citations (Scopus)

Decellularized Liver Matrix-Modified Cryogel Scaffolds as Potential Hepatocyte Carriers in Bioartificial Liver Support Systems and Implantable Liver Constructs

Damania, A., Kumar, A., Teotia, A. K., Kimura, H., Kamihira, M., Ijima, H., Sarin, S. K. & Kumar, A., Jan 10 2018, In : ACS Applied Materials and Interfaces. 10, 1, p. 114-126 13 p.

Research output: Contribution to journal › Article

Cryogels

Scaffolds (biology)

Liver

Bioreactors

Tissue engineering

2 Citations (Scopus)

Fabrication of liver-derived extracellular matrix nanofibers and functional evaluation in in vitro culture using primary hepatocytes

Bual, R., Kimura, H., Ikegami, Y., Shirakigawa, N. & Ijima, H., Dec 1 2018, In : Materialia. 4, p. 518-528 11 p.

Research output: Contribution to journal › Article

Nanofibers

Liver

Military electronic countermeasures

Scaffolds (biology)

Fabrication

2017

6 Citations (Scopus)

Alleviating liver failure conditions using an integrated hybrid cryogel based cellular bioreactor as a bioartificial liver support

Kumar, A., Damania, A., Hassan, M., Shirakigawa, N., Mizumoto, H., Kumar, A., Sarin, S. K., Ijima, H. & Kamihira, M., Jan 12 2017, In : Scientific reports. 7, 40323.

Research output: Contribution to journal › Article

Cryogels

Artificial Liver

Liver Failure

Bioreactors

Plasmapheresis

2 Citations (Scopus)

Decellularized tissue engineering

Shirakigawa, N. & Ijima, H., Jan 1 2017, Advanced Structured Materials. Springer Verlag, p. 185-226 42 p. (Advanced Structured Materials; vol. 66).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Tissue engineering

Tissue

Scaffolds (biology)

Cytokines

3 Citations (Scopus)

Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture

Nagai, T., Ikegami, Y., Mizumachi, H., Shirakigawa, N. & Ijima, H., Oct 1 2017, In : Journal of Bioscience and Bioengineering. 124, 4, p. 430-438 9 p.

Research output: Contribution to journal › Article

Cell culture

Extracellular Matrix

Gels

Hydrogel

Collagen

2016

1 Citation (Scopus)

Fetal liver cell-containing hybrid organoids improve cell viability and albumin production upon transplantation

Ye, J., Shirakigawa, N. & Ijima, H., Jun 1 2016, In : Journal of Bioscience and Bioengineering. 121, 6, p. 701-708 8 p.

Research output: Contribution to journal › Article

Organoids

Hybrid Cells

Liver

Albumins

Cell Survival

2015

4 Citations (Scopus)

Hybrid organoids consisting of extracellular matrix gel particles and hepatocytes for transplantation

Ye, J., Shirakigawa, N. & Ijima, H., Aug 1 2015, In : Journal of Bioscience and Bioengineering. 120, 2, p. 231-237 7 p.

Research output: Contribution to journal › Article

Organoids

Particles (particulate matter)

Extracellular Matrix

Hepatocytes

Gels

2014

15 Citations (Scopus)

Micropatterning electrospun scaffolds to create intrinsic vascular networks

Jeffries, E. M., Nakamura, S., Lee, K. W., Clampffer, J., Ijima, H. & Wang, Y., Nov 1 2014, In : Macromolecular Bioscience. 14, 11, p. 1514-1520 7 p.

Research output: Contribution to journal › Article

Scaffolds

Blood Vessels

Perfusion

Porosity

Tissue

Molecular structure of matrices solubilized with HCl from decellularized liver and the production of albumin from hepatocytes cultured on the matrices

Nakamura, S., Nishimura, S. & Ijima, H., Aug 17 2014, In : Transactions of Japanese Society for Medical and Biological Engineering. 52, p. O-194-O-195

Research output: Contribution to journal › Article

Military electronic countermeasures

Liver

Molecular structure

Collagen

Adhesives

2013

25 Citations (Scopus)

Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering

Shirakigawa, N., Takei, T. & Ijima, H., Dec 1 2013, In : Journal of Bioscience and Bioengineering. 116, 6, p. 740-745 6 p.

Research output: Contribution to journal › Article

Liver

Blood Vessels

Hepatocytes

Artificial Liver

Hepatic Veins

17 Citations (Scopus)

Heparin-conjugated gelatin as a growth factor immobilization scaffold

Nakamura, S., Kubo, T. & Ijima, H., May 1 2013, In : Journal of Bioscience and Bioengineering. 115, 5, p. 562-567 6 p.

Research output: Contribution to journal › Article

Gelatin

Scaffolds (biology)

Immobilization

Heparin

Intercellular Signaling Peptides and Proteins

1 Citation (Scopus)

Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats

Shirakigawa, N. & Ijima, H., May 1 2013, In : Journal of Bioscience and Bioengineering. 115, 5, p. 568-570 3 p.

Research output: Contribution to journal › Article

Hepatectomy

Liver

Rats

Tissue

Hydrogel

25 Citations (Scopus)

Solubilized matrix derived from decellularized liver as a growth factor-immobilizable scaffold for hepatocyte culture

Nakamura, S. & Ijima, H., Dec 1 2013, In : Journal of Bioscience and Bioengineering. 116, 6, p. 746-753 8 p.

Research output: Contribution to journal › Article

Military electronic countermeasures

Scaffolds (biology)

Liver

Hepatocyte Growth Factor

Hepatocytes

2012

12 Citations (Scopus)

A comparison of sodium sulfate, sodium phosphate, and boric acid for preparation of immobilized Pseudomonas putida F1 in poly(vinyl alcohol) beads

Takei, T., Ikeda, K., Ijima, H., Yoshida, M. & Kawakami, K., Jul 1 2012, In : Polymer Bulletin. 69, 3, p. 363-373 11 p.

Research output: Contribution to journal › Article

boric acids

Boric acid

Sodium sulfate

pseudomonas

sodium sulfates

45 Citations (Scopus)

Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering

Shirakigawa, N., Ijima, H. & Takei, T., Nov 1 2012, In : Journal of Bioscience and Bioengineering. 114, 5, p. 546-551 6 p.

Research output: Contribution to journal › Article

Tissue Engineering

Scaffolds (biology)

Tissue engineering

Liver

Blood Vessels

6 Citations (Scopus)

Development of growth factor-immobilizable material for hepatocyte transplantation

Hou, Y. T., Ijima, H., Shirakigawa, N., Takei, T. & Kawakami, K., Dec 15 2012, In : Biochemical Engineering Journal. 69, p. 172-181 10 p.

Research output: Contribution to journal › Article

Hepatocytes

Intercellular Signaling Peptides and Proteins

Transplantation

Heparin

Collagen

12 Citations (Scopus)

Evaluation of a hybrid artificial liver module based on a spheroid culture system of embryonic stem cell-derived hepatic cells

Mizumoto, H., Hayashi, S., Matsumoto, K., Ikeda, K., Kusumi, T., Inamori, M., Nakazawa, K., Ijima, H., Funatsu, K. & Kajiwara, T., Apr 18 2012, In : Cell Transplantation. 21, 2-3, p. 421-428 8 p.

Research output: Contribution to journal › Article

Artificial Liver

Embryonic Stem Cells

Stem cells

Cell culture

Liver

7 Citations (Scopus)

Fabrication of capillary-like network in a matrix of water-soluble polymer using poly(methyl methacrylate) microfibers

Takei, T., Kishihara, N., Ijima, H. & Kawakami, K., Feb 1 2012, In : Artificial Cells, Blood Substitutes, and Biotechnology. 40, 1-2, p. 66-69 4 p.

Research output: Contribution to journal › Article

Polymethyl Methacrylate

Polymethyl methacrylates

Polymers

Gels

Fabrication

51 Citations (Scopus)

Synthesis of a chitosan derivative soluble at neutral pH and gellable by freeze-thawing, and its application in wound care

Takei, T., Nakahara, H., Ijima, H. & Kawakami, K., Feb 1 2012, In : Acta Biomaterialia. 8, 2, p. 686-693 8 p.

Research output: Contribution to journal › Article

Thawing

Chitosan

Derivatives

Wounds and Injuries

Hydrogels

2011

3 Citations (Scopus)

An evaluation of the utility of the hepatic differentiation method using hollow fiber/organoid culture for the development of a hybrid artificial liver device

Amimoto, N., Mizumoto, H., Nakazawa, K., Ijima, H., Funatsu, K. & Kajiwara, T., Sep 15 2011, In : Biochemical Engineering Journal. 56, 1-2, p. 69-74 6 p.

Research output: Contribution to journal › Article

Organoids

Artificial Liver

Liver

Hepatocytes

Equipment and Supplies

60 Citations (Scopus)

Fabrication of poly(vinyl alcohol) hydrogel beads crosslinked using sodium sulfate for microorganism immobilization

Takei, T., Ikeda, K., Ijima, H. & Kawakami, K., Feb 1 2011, In : Process Biochemistry. 46, 2, p. 566-571 6 p.

Research output: Contribution to journal › Article

Boric acid

Sodium sulfate

Hydrogel

Hydrogels

Immobilization

31 Citations (Scopus)

Growth factor/heparin-immobilized collagen gel system enhances viability of transplanted hepatocytes and induces angiogenesis

Hou, Y. T., Ijima, H., Takei, T. & Kawakami, K., Sep 1 2011, In : Journal of Bioscience and Bioengineering. 112, 3, p. 265-272 8 p.

Research output: Contribution to journal › Article

Collagen

Heparin

Hepatocytes

Intercellular Signaling Peptides and Proteins

Gels

15 Citations (Scopus)

Hepatic differentiation of mouse embryonic stem cells and induced pluripotent stem cells during organoid formation in hollow fibers

Amimoto, N., Mizumoto, H., Nakazawa, K., Ijima, H., Funatsu, K. & Kajiwara, T., Aug 1 2011, In : Tissue Engineering - Part A. 17, 15-16, p. 2071-2078 8 p.

Research output: Contribution to journal › Article

Organoids

Induced Pluripotent Stem Cells

Stem cells

Artificial Liver

Fibers

20 Citations (Scopus)

In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications

Takei, T., Sugihara, K., Ijima, H. & Kawakami, K., Nov 1 2011, In : Journal of Bioscience and Bioengineering. 112, 5, p. 491-494 4 p.

Research output: Contribution to journal › Article

Sugar beets

Beta vulgaris

Enzymes

Horseradish Peroxidase

Gels

2010

4 Citations (Scopus)

Albumin production activity of primary rat hepatocytes is improved on type V collagen

Ijima, H., Ogata, R., Murasawa, Y. & Wang, P. C., Feb 1 2010, In : Journal of Bioscience and Bioengineering. 109, 2, p. 179-181 3 p.

Research output: Contribution to journal › Article

Collagen Type V

Collagen

Rats

Hepatocytes

Albumins

8 Citations (Scopus)

Development of hepatocyte-embedded hydrogel-filled macroporous scaffold cultures using transglutaminase

Ijima, H., Hou, Y. T. & Takei, T., Nov 15 2010, In : Biochemical Engineering Journal. 52, 2-3, p. 276-281 6 p.

Research output: Contribution to journal › Article

Transglutaminases

Hydrogel

Hydrogels

Scaffolds

Hepatocytes

27 Citations (Scopus)

Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes

Hou, Y. T., Ijima, H., Matsumoto, S., Kubo, T., Takei, T., Sakai, S. & Kawakami, K., Aug 1 2010, In : Journal of Bioscience and Bioengineering. 110, 2, p. 208-216 9 p.

Research output: Contribution to journal › Article

Hepatocyte Growth Factor

Collagen

Heparin

Hepatocytes

Albumins

41 Citations (Scopus)

In situ gellable oxidized citrus pectin for localized delivery of anticancer drugs and prevention of homotypic cancer cell aaggregation

Takei, T., Sato, M., Ijima, H. & Kawakami, K., Dec 13 2010, In : Biomacromolecules. 11, 12, p. 3525-3530 6 p.

Research output: Contribution to journal › Article

Hydrogels

Cells

Doxorubicin

Pharmaceutical Preparations

Drug delivery

14 Citations (Scopus)

Practical and functional culture technologies for primary hepatocytes

Ijima, H., Feb 15 2010, In : Biochemical Engineering Journal. 48, 3, p. 332-336 5 p.

Research output: Contribution to journal › Review article

Organoids

Liver

Hepatocytes

Technology

Metabolism

14 Citations (Scopus)

Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light

Mu, C., Sakai, S., Ijima, H. & Kawakami, K., Jun 1 2010, In : Journal of Bioscience and Bioengineering. 109, 6, p. 618-621 4 p.

Research output: Contribution to journal › Article

Photopolymerization

Alginate

Capsules

Irradiation

Light

20 Citations (Scopus)

Preparation of polymeric microcapsules enclosing microbial cells by radical suspension polymerization via water-in-oil-in-water emulsion

Takei, T., Ikeda, K., Ijima, H., Kawakami, K., Yoshida, M. & Hatate, Y., Jul 1 2010, In : Polymer Bulletin. 65, 3, p. 283-291 9 p.

Research output: Contribution to journal › Article

Emulsions

Yeast

emulsions

Capsules

Suspensions

2009

10 Citations (Scopus)

Composition of culture medium is more important than co-culture with hepatic non-parenchymal cells in albumin production activity of primary rat hepatocytes, and the effect was enhanced by hepatocytes spheroid culture in collagen gel

Ijima, H., Kakeya, Y., Yokonuma, T., Hou, Y. T. & Takei, T., Aug 15 2009, In : Biochemical Engineering Journal. 45, 3, p. 226-231 6 p.

Research output: Contribution to journal › Article

Coculture Techniques

Collagen

Culture Media

Rats

Hepatocytes

2 Citations (Scopus)

Development of a practical small-scale circulation bioreactor and application to a drug metabolism simulator

Ijima, H., Kakeya, Y., Ogata, T. & Sakai, T., May 15 2009, In : Biochemical Engineering Journal. 44, 2-3, p. 292-296 5 p.

Research output: Contribution to journal › Article

Bioreactors

Metabolism

Simulators

Hepatocytes

Lidocaine

7 Citations (Scopus)

Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes

Kusumi, T., Ishihara, K., Mizumoto, H., Nakazawa, K., Ijima, H., Funatsu, K. & Kajiwara, T., May 1 2009, In : Journal of Bioscience and Bioengineering. 107, 5, p. 552-555 4 p.

Research output: Contribution to journal › Article

Organoids

Bioreactors

Hepatocytes

Oxygen supply

polycarbonate

12 Citations (Scopus)

Hepatocyte growth factor and epidermal growth factor promote spheroid formation in polyurethane foam/hepatocyte culture and improve expression and maintenance of albumin production

Ijima, H., Mizumoto, H., Nakazawa, K., Kajiwara, T., Matsushita, T. & Funatsu, K., Dec 1 2009, In : Biochemical Engineering Journal. 47, 1-3, p. 19-26 8 p.

Research output: Contribution to journal › Article

Hepatocyte Growth Factor

Epidermal Growth Factor

Polyurethanes

Foams

Hepatocytes

13 Citations (Scopus)

Magnetic separation of cells in coculture systems using magnetite cationic liposomes.

Ito, A., Jitsunobu, H., Yoshinori, K., Ijima, H. & Kamihira, M., Jan 1 2009, In : Tissue engineering. Part C, Methods. 15, 3, p. 413-423 11 p.

Research output: Contribution to journal › Article

Ferrosoferric Oxide

Magnetic separation

Cell Separation

Liposomes

Magnetite

16 Citations (Scopus)

Primary rat hepatocytes form spheroids on hepatocyte growth factor/heparin-immobilized collagen film and maintain high albumin production

Ijima, H., Kubo, T. & Hou, Y. T., Oct 1 2009, In : Biochemical Engineering Journal. 46, 2, p. 227-233 7 p.

Research output: Contribution to journal › Article

Hepatocyte Growth Factor

Collagen

Heparin

Rats

Hepatocytes

2008

24 Citations (Scopus)

A new culture technique for hepatocyte organoid formation and long-term maintenance of liver-specific functions

Mizumoto, H., Ishihara, K., Nakazawa, K., Ijima, H., Funatsu, K. & Kajiwara, T., Jan 1 2008, In : Tissue Engineering - Part C: Methods. 14, 2, p. 167-175 9 p.

Research output: Contribution to journal › Article

Organoids

Culture Techniques

Liver

Hepatocytes

Artificial Liver

11 Citations (Scopus)

Enhancement of cell function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells

Ito, A., Kiyohara, T., Yoshinori, K., Ijima, H. & Kamihira, M., Jun 1 2008, In : Journal of Bioscience and Bioengineering. 105, 6, p. 679-682 4 p.

Research output: Contribution to journal › Article

Cadherins

Fibroblasts

Cell Communication

Hepatocytes

Cell adhesion

Formation of Co-cultured hepatocyte spheroid which aimed at liver tissue engineering, and preliminary study for the device

Ijima, H., Kakeya, Y., Ogata, R., Hou, Y. T., Kubo, T., Sakai, S. & Kawakami, K., Dec 1 2008, 8th World Biomaterials Congress 2008. 1 p. (8th World Biomaterials Congress 2008; vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tissue engineering

Liver

Hepatocytes

18 Citations (Scopus)

Hepatic Differentiation of Embryonic Stem Cells in HF/Organoid Culture

Mizumoto, H., Aoki, K., Nakazawa, K., Ijima, H., Funatsu, K. & Kajiwara, T., Mar 1 2008, In : Transplantation Proceedings. 40, 2, p. 611-613 3 p.

Research output: Contribution to journal › Article

Organoids

Embryonic Stem Cells

Liver

Hepatocytes

Macaca fascicularis