αB-crystallin and 27-kd heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers

T. Iwaki, A. Iwaki, J. Tateishi, Y. Sakaki, J. E. Goldman

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157 Citations (Scopus)

Abstract

To understand the significance of the accumulation of αB-crystallin in Rosenthal fibers within astrocytes, the expression and metabolism of αB- crystallin in glioma cell lines were examined under the conditions of heat and oxidative stress, αB-crystallin mRNA was increased after both stresses, and αB-crystallin protein moved from a detergent-soluble form. In addition, Western blotting of Alexander's disease brain homogenates revealed that the 27-kd heat shock protein (HSP27), which is related to αB-crystallin, accumulates along with αB-crystallin. The presence of HSP27 in Rosenthal fibers was directly demonstrated by immunohistochemistry. Our results suggest that astrocytes in Alexander's disease may be involved in an as yet unknown kind of stress reaction that causes the accumulation of αB-crystallin and HSP27 and results in Rosenthal fiber formation.

Original languageEnglish
Pages (from-to)487-495
Number of pages9
JournalAmerican Journal of Pathology
Volume143
Issue number2
Publication statusPublished - 1993

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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