TY - JOUR
T1 - αB-Crystallin in C6 glioma cells supports their survival in elevated extracellular K+
T2 - the implication of a protective role of αB-crystallin accumulation in reactive glia
AU - Iwaki, Toru
AU - Iwaki, Akiko
AU - Fukumaki, Yasuyuki
AU - Tateishi, Jun
N1 - Funding Information:
Supported by a Grant-in-Aid for General Scientific Research from the Ministry of Education, Science and Culture of Japan (06454694).
PY - 1995/2/27
Y1 - 1995/2/27
N2 - It has been shown by immunohistochemical studies that αB-crystallin accumulates in the reactive and neoplastic glial cells in a variety of pathologic situations. However, the molecular mechanism for the induction of αB-crystallin in diseased brains is still unknown. Since any destructive brain lesions cause an abnormal elevation in the potassium (K+) concentration of the extracellular space, which disturbs the regulatory mechanism of glial cell volume, we investigated the influence of elevated extracellular K+ on the expression of αB-crystallin in glial cells. The treatment of rat C6 glioma cells with augmented K+ in the culture media induced an accumulation of αB-crystallin mRNA in a dose-dependent manner and an accumulation of the αB-crystallin as well. Furthermore, an overexpression of αB-crystallin in the C6 transformant transfected with a rat αB-crystallin cDNA conferred a resistant phenotype against the insult of elevated extracellular K+ on the glioma cells. Thus, αB-crystallin may contribute to the survival of reactive glia in the presence of a high extracellular K+ concentration.
AB - It has been shown by immunohistochemical studies that αB-crystallin accumulates in the reactive and neoplastic glial cells in a variety of pathologic situations. However, the molecular mechanism for the induction of αB-crystallin in diseased brains is still unknown. Since any destructive brain lesions cause an abnormal elevation in the potassium (K+) concentration of the extracellular space, which disturbs the regulatory mechanism of glial cell volume, we investigated the influence of elevated extracellular K+ on the expression of αB-crystallin in glial cells. The treatment of rat C6 glioma cells with augmented K+ in the culture media induced an accumulation of αB-crystallin mRNA in a dose-dependent manner and an accumulation of the αB-crystallin as well. Furthermore, an overexpression of αB-crystallin in the C6 transformant transfected with a rat αB-crystallin cDNA conferred a resistant phenotype against the insult of elevated extracellular K+ on the glioma cells. Thus, αB-crystallin may contribute to the survival of reactive glia in the presence of a high extracellular K+ concentration.
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U2 - 10.1016/0006-8993(94)01393-V
DO - 10.1016/0006-8993(94)01393-V
M3 - Article
C2 - 7757478
AN - SCOPUS:0028984955
SN - 0006-8993
VL - 673
SP - 47
EP - 52
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -