αB-Crystallin in C6 glioma cells supports their survival in elevated extracellular K+: the implication of a protective role of αB-crystallin accumulation in reactive glia

Toru Iwaki, Akiko Iwaki, Yasuyuki Fukumaki, Jun Tateishi

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Abstract

It has been shown by immunohistochemical studies that αB-crystallin accumulates in the reactive and neoplastic glial cells in a variety of pathologic situations. However, the molecular mechanism for the induction of αB-crystallin in diseased brains is still unknown. Since any destructive brain lesions cause an abnormal elevation in the potassium (K+) concentration of the extracellular space, which disturbs the regulatory mechanism of glial cell volume, we investigated the influence of elevated extracellular K+ on the expression of αB-crystallin in glial cells. The treatment of rat C6 glioma cells with augmented K+ in the culture media induced an accumulation of αB-crystallin mRNA in a dose-dependent manner and an accumulation of the αB-crystallin as well. Furthermore, an overexpression of αB-crystallin in the C6 transformant transfected with a rat αB-crystallin cDNA conferred a resistant phenotype against the insult of elevated extracellular K+ on the glioma cells. Thus, αB-crystallin may contribute to the survival of reactive glia in the presence of a high extracellular K+ concentration.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalBrain Research
Volume673
Issue number1
DOIs
Publication statusPublished - Feb 27 1995

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Crystallins
Glioma
Neuroglia
Extracellular Space
Brain Diseases
Cell Size
Culture Media
Potassium
Complementary DNA
Phenotype
Messenger RNA
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

αB-Crystallin in C6 glioma cells supports their survival in elevated extracellular K+ : the implication of a protective role of αB-crystallin accumulation in reactive glia. / Iwaki, Toru; Iwaki, Akiko; Fukumaki, Yasuyuki; Tateishi, Jun.

In: Brain Research, Vol. 673, No. 1, 27.02.1995, p. 47-52.

Research output: Contribution to journalArticle

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