αB-crystallin in oxidative muscle fibers and its accumulation in ragged-red fibers: a comparative immunohistochemical and histochemical study in human skeletal muscle

Toru Iwaki, Akiko Iwaki, James E. Goldman

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The αB-crystallin gene is abundantly experssed in the vertebrate lens and at lower levels in various non-lenticular tissues. Among the non-lenticular tissues, αB-crystallin is present at high levels in the heart and skeletal muscle. Using a specific antibody against αB-crystallin, the cellular localization of αB-crystallin was studied in biopsies of human skeletal muscles. Expression of αB-crystallin was observed in normal oxidative muscle fibers that show positive reactions for NADH-tetrazolium reductase and cytochrome c oxidase. In muscle diseases increased immunoreactivity for αB-crystallin was found in ragged-red fibers, which stained darkly with histochemistry for succinate dehydrogenase. Since αB-crystallin is related to small heat-shock proteins and can be induced by various stress conditions, the increased αB-crystallin immunoreactivity of ragged-red fibers could result from profound oxidative stress produced by the abnormal mitochondrial metabolism.

Original languageEnglish
Pages (from-to)475-480
Number of pages6
JournalActa neuropathologica
Volume85
Issue number5
DOIs
Publication statusPublished - Apr 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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