αB-crystallin is expressed in non-lenticular tissues and accumulates in Alexander's disease brain

Toru Iwaki, Akiko Kume-Iwaki, Ronald K.H. Liem, James E. Goldman

Research output: Contribution to journalArticle

466 Citations (Scopus)

Abstract

Rosenthal fibers (RFs) are abnormal inclusions within astrocytes, characteristic of Alexander's disease. We have previously isolated a 22 kd protein component of RFs from Alexander's disease brain. By Western blotting, we detected its equivalent in several rat organs, with the highest level in heart, and in a human astrocytoma cell line (U-373MG). A cDNA library established from U-373MG was screened with an anti-RF protein antibody. A partial cDNA clone encoding the lens protein αB-crystallin was isolated. The anti-RF protein antibodies react with lens αB-crystallin. Furthermore, the distribution of αB-crystallin mRNA in rat organs is consistent with the Western blots. Therefore, αB-crystallin is not lens-specific and it can accumulate in large amounts in astrocytes in pathological conditions.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalCell
Volume57
Issue number1
DOIs
Publication statusPublished - Apr 7 1989
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this