TY - JOUR
T1 - αB-crystallin is expressed in non-lenticular tissues and accumulates in Alexander's disease brain
AU - Iwaki, Toru
AU - Kume-Iwaki, Akiko
AU - Liem, Ronald K.H.
AU - Goldman, James E.
N1 - Funding Information:
We are grateful to A. Spector, Ft. Chiesa, and M. McDermott for providing the bovine lens a-crystallin and for helpful discussions, and J. Tateishi for providing the frozen Alexander’s tissue. We thank S. S. M Chin for help with the computer search, E. Corbin for technical assistance, and M. A. Gawinowicz-Kolks for the amino acid analysis. This work was supported by NINCDS Javits Neuroscientist Awards, NSl7l25 (J. E. G) and NS15182 (R. K. H. L.).
PY - 1989/4/7
Y1 - 1989/4/7
N2 - Rosenthal fibers (RFs) are abnormal inclusions within astrocytes, characteristic of Alexander's disease. We have previously isolated a 22 kd protein component of RFs from Alexander's disease brain. By Western blotting, we detected its equivalent in several rat organs, with the highest level in heart, and in a human astrocytoma cell line (U-373MG). A cDNA library established from U-373MG was screened with an anti-RF protein antibody. A partial cDNA clone encoding the lens protein αB-crystallin was isolated. The anti-RF protein antibodies react with lens αB-crystallin. Furthermore, the distribution of αB-crystallin mRNA in rat organs is consistent with the Western blots. Therefore, αB-crystallin is not lens-specific and it can accumulate in large amounts in astrocytes in pathological conditions.
AB - Rosenthal fibers (RFs) are abnormal inclusions within astrocytes, characteristic of Alexander's disease. We have previously isolated a 22 kd protein component of RFs from Alexander's disease brain. By Western blotting, we detected its equivalent in several rat organs, with the highest level in heart, and in a human astrocytoma cell line (U-373MG). A cDNA library established from U-373MG was screened with an anti-RF protein antibody. A partial cDNA clone encoding the lens protein αB-crystallin was isolated. The anti-RF protein antibodies react with lens αB-crystallin. Furthermore, the distribution of αB-crystallin mRNA in rat organs is consistent with the Western blots. Therefore, αB-crystallin is not lens-specific and it can accumulate in large amounts in astrocytes in pathological conditions.
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U2 - 10.1016/0092-8674(89)90173-6
DO - 10.1016/0092-8674(89)90173-6
M3 - Article
C2 - 2539261
AN - SCOPUS:0024521440
SN - 0092-8674
VL - 57
SP - 71
EP - 78
JO - Cell
JF - Cell
IS - 1
ER -
