β-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis

Yves Hatzfeld, Akiko Maruyama, Ahlert Schmidt, Masaaki Noji, Kimiharu Ishizawa, Kazuki Saito

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

β-Cyano-alanine synthase (CAS; EC 4.4.1.9) plays an important role in cyanide metabolism in plants. Although the enzymatic activity of β-cyano-Ala synthase has been detected in a variety of plants, no cDNA or gene has been identified so far. We hypothesized that the mitochondrial cysteine synthase (CS; EC 4.2.99.8) isoform, Bsas3, could actually be identical to CAS in spinach (Spinacia oleracea) and Arabidopsis. An Arabidopsis expressed sequence tag database was searched for putative Bsas3 homologs and four new CS-like isoforms, ARAth;Bsas1;1, ARAth;Bsas3;1, ARAth;Bsas4;1, and ARAth;Bsas4;2, were identified in the process. ARAth;Bsas3;1 protein was homologous to the mitochondrial SPIol;Bsas3;1 isoform from spinach, whereas ARAth;Bsas4;1 and ARAth;Bsas4;2 proteins defined a new class within the CS-like proteins family. In contrast to spinach SPIol;Bsas1;1 and SPIol;Bsas2;1 recombinant proteins, spinach SPIol;Bsas3;1 and Arabidopsis ARAth; Bsas3;1 recombinant proteins exhibited preferred substrate specificities for the CAS reaction rather than for the CS reaction, which identified these Bsas3 isoforms as CAS. Immunoblot studies supported this conclusion. This is the first report of the identification of CAS synthase-encoding cDNAs in a living organism. A new nomenclature for CS-like proteins in plants is also proposed.

Original languageEnglish
Pages (from-to)1163-1171
Number of pages9
JournalPlant physiology
Volume123
Issue number3
Publication statusPublished - Jul 1 2000
Externally publishedYes

Fingerprint

Cysteine Synthase
Spinacia oleracea
Arabidopsis
spinach
Protein Isoforms
recombinant proteins
beta-cyanoalanine synthase
Proteins
Recombinant Proteins
proteins
Complementary DNA
cyanides
substrate specificity
plant proteins
expressed sequence tags
Plant Proteins
alanine
Expressed Sequence Tags
Cyanides
Substrate Specificity

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Hatzfeld, Y., Maruyama, A., Schmidt, A., Noji, M., Ishizawa, K., & Saito, K. (2000). β-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis. Plant physiology, 123(3), 1163-1171.

β-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis. / Hatzfeld, Yves; Maruyama, Akiko; Schmidt, Ahlert; Noji, Masaaki; Ishizawa, Kimiharu; Saito, Kazuki.

In: Plant physiology, Vol. 123, No. 3, 01.07.2000, p. 1163-1171.

Research output: Contribution to journalArticle

Hatzfeld, Y, Maruyama, A, Schmidt, A, Noji, M, Ishizawa, K & Saito, K 2000, 'β-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis', Plant physiology, vol. 123, no. 3, pp. 1163-1171.
Hatzfeld, Yves ; Maruyama, Akiko ; Schmidt, Ahlert ; Noji, Masaaki ; Ishizawa, Kimiharu ; Saito, Kazuki. / β-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis. In: Plant physiology. 2000 ; Vol. 123, No. 3. pp. 1163-1171.
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