β-Eliminative Cleavage of the Acidic Polysaccharide of Fusarium sp. M7-1 by an Enzyme Preparation of Cellulomonas sp

Shojiro Iwahara; Takayuki Jikibara; Satoshi Miki; Kaoru Takegawa

doi:10.1271/bbb1961.54.419

β-Eliminative Cleavage of the Acidic Polysaccharide of Fusarium sp. M7-1 by an Enzyme Preparation of Cellulomonas sp

Shojiro Iwahara, Takayuki Jikibara, Satoshi Miki, Kaoru Takegawa

Research output: Contribution to journal › Article › peer-review

Abstract

By digestion with an enzyme preparation derived from a culture filtrate of Cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and β1 → 2mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of Fusarium sp. M7-1 by acetolysis. The unsaturated disaccharide produced was isolated and identified as 4-deoxy-L-threo-hex-4-enopyranouronosyl α(1 → 2)-D-galactose. The same unsaturated sugar linked to the polysaccharide was also produced accompanied by the release of mannose and β → 2mannobiose from the acidic polysaccharide by the enzyme digestion.

Original language	English
Pages (from-to)	419-425
Number of pages	7
Journal	Agricultural and Biological Chemistry
Volume	54
Issue number	2
DOIs	https://doi.org/10.1271/bbb1961.54.419
Publication status	Published - 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1271/bbb1961.54.419

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title = "β-Eliminative Cleavage of the Acidic Polysaccharide of Fusarium sp. M7-1 by an Enzyme Preparation of Cellulomonas sp",

abstract = "By digestion with an enzyme preparation derived from a culture filtrate of Cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and β1 → 2mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of Fusarium sp. M7-1 by acetolysis. The unsaturated disaccharide produced was isolated and identified as 4-deoxy-L-threo-hex-4-enopyranouronosyl α(1 → 2)-D-galactose. The same unsaturated sugar linked to the polysaccharide was also produced accompanied by the release of mannose and β → 2mannobiose from the acidic polysaccharide by the enzyme digestion.",

author = "Shojiro Iwahara and Takayuki Jikibara and Satoshi Miki and Kaoru Takegawa",

note = "Funding Information: t A part of this study was presented at the Annual Meeting of Kansai Division of the Agricultural Chemical Society of Japan, Takamatsu. October 1988. * Corresponding author.",

year = "1990",

doi = "10.1271/bbb1961.54.419",

language = "English",

volume = "54",

pages = "419--425",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

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T1 - β-Eliminative Cleavage of the Acidic Polysaccharide of Fusarium sp. M7-1 by an Enzyme Preparation of Cellulomonas sp

AU - Iwahara, Shojiro

AU - Jikibara, Takayuki

AU - Miki, Satoshi

AU - Takegawa, Kaoru

N1 - Funding Information: t A part of this study was presented at the Annual Meeting of Kansai Division of the Agricultural Chemical Society of Japan, Takamatsu. October 1988. * Corresponding author.

PY - 1990

Y1 - 1990

N2 - By digestion with an enzyme preparation derived from a culture filtrate of Cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and β1 → 2mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of Fusarium sp. M7-1 by acetolysis. The unsaturated disaccharide produced was isolated and identified as 4-deoxy-L-threo-hex-4-enopyranouronosyl α(1 → 2)-D-galactose. The same unsaturated sugar linked to the polysaccharide was also produced accompanied by the release of mannose and β → 2mannobiose from the acidic polysaccharide by the enzyme digestion.

AB - By digestion with an enzyme preparation derived from a culture filtrate of Cellulomonas sp., an unsaturated disaccharide was produced accompanied by the release of mannose and β1 → 2mannobiose from the acidic oligomer mixture that was obtained from the acidic polysaccharide of Fusarium sp. M7-1 by acetolysis. The unsaturated disaccharide produced was isolated and identified as 4-deoxy-L-threo-hex-4-enopyranouronosyl α(1 → 2)-D-galactose. The same unsaturated sugar linked to the polysaccharide was also produced accompanied by the release of mannose and β → 2mannobiose from the acidic polysaccharide by the enzyme digestion.

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JO - Bioscience, Biotechnology and Biochemistry

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β-Eliminative Cleavage of the Acidic Polysaccharide of Fusarium sp. M7-1 by an Enzyme Preparation of Cellulomonas sp

Abstract

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