β2-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro

Miyako Takata; Naoto Burioka; Shigehiro Ohdo; Yasushi Fukuoka; Masanori Miyata; Masahiro Endo; Hisashi Suyama; Eiji Shimizu

doi:10.1080/07420520500179167

β₂-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro

Miyako Takata, Naoto Burioka, Shigehiro Ohdo, Yasushi Fukuoka, Masanori Miyata, Masahiro Endo, Hisashi Suyama, Eiji Shimizu

Pharmaceutical Health Care and Sciences Faculty

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the β₂-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of β₂-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. β²-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.

Original language	English
Pages (from-to)	777-783
Number of pages	7
Journal	Chronobiology International
Volume	22
Issue number	4
DOIs	https://doi.org/10.1080/07420520500179167
Publication status	Published - 2005

All Science Journal Classification (ASJC) codes

Physiology
Physiology (medical)

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10.1080/07420520500179167

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β₂-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro. / Takata, Miyako; Burioka, Naoto; Ohdo, Shigehiro; Fukuoka, Yasushi; Miyata, Masanori; Endo, Masahiro; Suyama, Hisashi; Shimizu, Eiji.

In: Chronobiology International, Vol. 22, No. 4, 2005, p. 777-783.

Research output: Contribution to journal › Article › peer-review

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title = "β2-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro",

abstract = "The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the β2-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of β2-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. β2-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.",

author = "Miyako Takata and Naoto Burioka and Shigehiro Ohdo and Yasushi Fukuoka and Masanori Miyata and Masahiro Endo and Hisashi Suyama and Eiji Shimizu",

note = "Funding Information: We thank Drs. Kazuhiro Yagita and Hitoshi Okamura, Department of Anatomy and Brain Science, Kobe University School of Medicine, for their useful suggestion for immunoblot analysis. This study was partly supported by the grant (17590791) of the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

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AU - Takata, Miyako

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AU - Ohdo, Shigehiro

AU - Fukuoka, Yasushi

AU - Miyata, Masanori

AU - Endo, Masahiro

AU - Suyama, Hisashi

AU - Shimizu, Eiji

N1 - Funding Information: We thank Drs. Kazuhiro Yagita and Hitoshi Okamura, Department of Anatomy and Brain Science, Kobe University School of Medicine, for their useful suggestion for immunoblot analysis. This study was partly supported by the grant (17590791) of the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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AB - The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the β2-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of β2-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. β2-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.

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