TY - JOUR
T1 - β2-adrenoceptor agonists induce the mammalian clock gene, hPer1, mRNA in cultured human bronchial epithelium cells in vitro
AU - Takata, Miyako
AU - Burioka, Naoto
AU - Ohdo, Shigehiro
AU - Fukuoka, Yasushi
AU - Miyata, Masanori
AU - Endo, Masahiro
AU - Suyama, Hisashi
AU - Shimizu, Eiji
N1 - Funding Information:
We thank Drs. Kazuhiro Yagita and Hitoshi Okamura, Department of Anatomy and Brain Science, Kobe University School of Medicine, for their useful suggestion for immunoblot analysis. This study was partly supported by the grant (17590791) of the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the β2-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of β2-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. β2-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.
AB - The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the β2-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of β2-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. β2-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.
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U2 - 10.1080/07420520500179167
DO - 10.1080/07420520500179167
M3 - Article
C2 - 16147906
AN - SCOPUS:25444439285
VL - 22
SP - 777
EP - 783
JO - Chronobiology International
JF - Chronobiology International
SN - 0742-0528
IS - 4
ER -