TY - JOUR
T1 - Δ 9 -Tetrahydrocannabinol elicited 22-kHz ultrasonic vocalization changes after air puff stimulus through CB 1 receptor in adult rats
AU - Myose, Takayuki
AU - Shirakawa, Atsunori
AU - Irie, Keiichi
AU - Yamashita, Yuta
AU - Nakano, Takafumi
AU - Takase, Yumi
AU - Matsuo, Koichi
AU - Satho, Tomomitsu
AU - Tuchihashi, Ryota
AU - Kinjo, Junei
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
AU - Funada, Masahiko
AU - Sano, Kazunori
AU - Mishima, Kenichi
N1 - Funding Information:
This work was supported by funds (No.: 186006 ) from the Central Research Institute of Fukuoka University in japan .
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/5/14
Y1 - 2019/5/14
N2 - Δ 9 -Tetrahydrocannabinol (THC) is known to have various pharmacological effects mediated through activation of cannabinoid CB 1 and CB 2 receptors in rodents. In adult rats, 22- and 50-kHz ultrasonic vocalizations (USVs) serve as an effective communication system and as indicators of negative and positive states, respectively. The present study was performed to determine whether THC affects USVs in adult rats, and to determine the roles of cannabinoid receptors in these effects. THC (1, 3 mg/kg) was administered intraperitoneally to adult male Wistar rats 60 min before measurement of USVs. The CB 1 antagonist, SR141716 (3, 6 mg/kg), or CB 2 antagonist, AM630 (1, 10 mg/kg), was administered intraperitoneally 10 min before THC. USVs were measured during a 5-minute period without air puff stimulus or with air puff stimulus. THC did not affect 22- or 50-kHz USVs without air puff stimulus. On the other hand, THC significantly increased the number of 22-kHz USVs, but not 50-kHz USVs, after air puff stimulus. Moreover, SR141716 at 6 mg/kg, but not AM630 at either dose, inhibited the increase in number of 22-kHz USVs induced by THC after air puff stimulus. These results suggest that THC induced changes in sensitivity to aversive air puff stimuli through CB 1 receptors, and as a result increased emission of 22-kHz USVs in rats.
AB - Δ 9 -Tetrahydrocannabinol (THC) is known to have various pharmacological effects mediated through activation of cannabinoid CB 1 and CB 2 receptors in rodents. In adult rats, 22- and 50-kHz ultrasonic vocalizations (USVs) serve as an effective communication system and as indicators of negative and positive states, respectively. The present study was performed to determine whether THC affects USVs in adult rats, and to determine the roles of cannabinoid receptors in these effects. THC (1, 3 mg/kg) was administered intraperitoneally to adult male Wistar rats 60 min before measurement of USVs. The CB 1 antagonist, SR141716 (3, 6 mg/kg), or CB 2 antagonist, AM630 (1, 10 mg/kg), was administered intraperitoneally 10 min before THC. USVs were measured during a 5-minute period without air puff stimulus or with air puff stimulus. THC did not affect 22- or 50-kHz USVs without air puff stimulus. On the other hand, THC significantly increased the number of 22-kHz USVs, but not 50-kHz USVs, after air puff stimulus. Moreover, SR141716 at 6 mg/kg, but not AM630 at either dose, inhibited the increase in number of 22-kHz USVs induced by THC after air puff stimulus. These results suggest that THC induced changes in sensitivity to aversive air puff stimuli through CB 1 receptors, and as a result increased emission of 22-kHz USVs in rats.
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U2 - 10.1016/j.neulet.2019.02.010
DO - 10.1016/j.neulet.2019.02.010
M3 - Article
C2 - 30742938
AN - SCOPUS:85062212664
SN - 0304-3940
VL - 701
SP - 132
EP - 135
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -