Δ 9 -Tetrahydrocannabinol elicited 22-kHz ultrasonic vocalization changes after air puff stimulus through CB 1 receptor in adult rats

Takayuki Myose, Atsunori Shirakawa, Keiichi Irie, Yuta Yamashita, Takafumi Nakano, Yumi Takase, Koichi Matsuo, Tomomitsu Satho, Ryota Tuchihashi, Junei Kinjo, Hiroyuki Tanaka, Satoshi Morimoto, Masahiko Funada, Kazunori Sano, Kenichi Mishima

Research output: Contribution to journalArticle

Abstract

Δ 9 -Tetrahydrocannabinol (THC) is known to have various pharmacological effects mediated through activation of cannabinoid CB 1 and CB 2 receptors in rodents. In adult rats, 22- and 50-kHz ultrasonic vocalizations (USVs) serve as an effective communication system and as indicators of negative and positive states, respectively. The present study was performed to determine whether THC affects USVs in adult rats, and to determine the roles of cannabinoid receptors in these effects. THC (1, 3 mg/kg) was administered intraperitoneally to adult male Wistar rats 60 min before measurement of USVs. The CB 1 antagonist, SR141716 (3, 6 mg/kg), or CB 2 antagonist, AM630 (1, 10 mg/kg), was administered intraperitoneally 10 min before THC. USVs were measured during a 5-minute period without air puff stimulus or with air puff stimulus. THC did not affect 22- or 50-kHz USVs without air puff stimulus. On the other hand, THC significantly increased the number of 22-kHz USVs, but not 50-kHz USVs, after air puff stimulus. Moreover, SR141716 at 6 mg/kg, but not AM630 at either dose, inhibited the increase in number of 22-kHz USVs induced by THC after air puff stimulus. These results suggest that THC induced changes in sensitivity to aversive air puff stimuli through CB 1 receptors, and as a result increased emission of 22-kHz USVs in rats.

Original languageEnglish
Pages (from-to)132-135
Number of pages4
JournalNeuroscience Letters
Volume701
DOIs
Publication statusPublished - May 14 2019

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Dronabinol
Ultrasonics
Air
rimonabant
Cannabinoid Receptors
Cannabinoids
Wistar Rats
Rodentia
Communication
Pharmacology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Δ 9 -Tetrahydrocannabinol elicited 22-kHz ultrasonic vocalization changes after air puff stimulus through CB 1 receptor in adult rats . / Myose, Takayuki; Shirakawa, Atsunori; Irie, Keiichi; Yamashita, Yuta; Nakano, Takafumi; Takase, Yumi; Matsuo, Koichi; Satho, Tomomitsu; Tuchihashi, Ryota; Kinjo, Junei; Tanaka, Hiroyuki; Morimoto, Satoshi; Funada, Masahiko; Sano, Kazunori; Mishima, Kenichi.

In: Neuroscience Letters, Vol. 701, 14.05.2019, p. 132-135.

Research output: Contribution to journalArticle

Myose, T, Shirakawa, A, Irie, K, Yamashita, Y, Nakano, T, Takase, Y, Matsuo, K, Satho, T, Tuchihashi, R, Kinjo, J, Tanaka, H, Morimoto, S, Funada, M, Sano, K & Mishima, K 2019, ' Δ 9 -Tetrahydrocannabinol elicited 22-kHz ultrasonic vocalization changes after air puff stimulus through CB 1 receptor in adult rats ', Neuroscience Letters, vol. 701, pp. 132-135. https://doi.org/10.1016/j.neulet.2019.02.010
Myose, Takayuki ; Shirakawa, Atsunori ; Irie, Keiichi ; Yamashita, Yuta ; Nakano, Takafumi ; Takase, Yumi ; Matsuo, Koichi ; Satho, Tomomitsu ; Tuchihashi, Ryota ; Kinjo, Junei ; Tanaka, Hiroyuki ; Morimoto, Satoshi ; Funada, Masahiko ; Sano, Kazunori ; Mishima, Kenichi. / Δ 9 -Tetrahydrocannabinol elicited 22-kHz ultrasonic vocalization changes after air puff stimulus through CB 1 receptor in adult rats In: Neuroscience Letters. 2019 ; Vol. 701. pp. 132-135.
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AU - Myose, Takayuki

AU - Shirakawa, Atsunori

AU - Irie, Keiichi

AU - Yamashita, Yuta

AU - Nakano, Takafumi

AU - Takase, Yumi

AU - Matsuo, Koichi

AU - Satho, Tomomitsu

AU - Tuchihashi, Ryota

AU - Kinjo, Junei

AU - Tanaka, Hiroyuki

AU - Morimoto, Satoshi

AU - Funada, Masahiko

AU - Sano, Kazunori

AU - Mishima, Kenichi

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N2 - Δ 9 -Tetrahydrocannabinol (THC) is known to have various pharmacological effects mediated through activation of cannabinoid CB 1 and CB 2 receptors in rodents. In adult rats, 22- and 50-kHz ultrasonic vocalizations (USVs) serve as an effective communication system and as indicators of negative and positive states, respectively. The present study was performed to determine whether THC affects USVs in adult rats, and to determine the roles of cannabinoid receptors in these effects. THC (1, 3 mg/kg) was administered intraperitoneally to adult male Wistar rats 60 min before measurement of USVs. The CB 1 antagonist, SR141716 (3, 6 mg/kg), or CB 2 antagonist, AM630 (1, 10 mg/kg), was administered intraperitoneally 10 min before THC. USVs were measured during a 5-minute period without air puff stimulus or with air puff stimulus. THC did not affect 22- or 50-kHz USVs without air puff stimulus. On the other hand, THC significantly increased the number of 22-kHz USVs, but not 50-kHz USVs, after air puff stimulus. Moreover, SR141716 at 6 mg/kg, but not AM630 at either dose, inhibited the increase in number of 22-kHz USVs induced by THC after air puff stimulus. These results suggest that THC induced changes in sensitivity to aversive air puff stimuli through CB 1 receptors, and as a result increased emission of 22-kHz USVs in rats.

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