cDNA encoding the 20-kD subunit of coatomer, ζ-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to ζ-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP- coated vesicles on Golgi cisternae. Unlike other coatomer subunits (β-, β'- , γ-, and ε-COP), ζ-COP exists in both coatomer bound and free pools.
All Science Journal Classification (ASJC) codes
- Cell Biology