λ DNA-Fragmenting Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+

Kazuki Shinohara, Masayasu So, Michiko Nonaka, Kazuo Nishiyama, Hiroki Murakami, Hirohisa Omura

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12 Citations (Scopus)

Abstract

Lambda DNA-fragmenting actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ were studied. The mixture of AsA or TR and Cu2+ caused a marked fragmentation of IDNA (3.2x107 daltons) within the first 1 min of reaction. Further incubation resulted in accumulation of the most abundant species of fragmented ADNA having a molecular weight of 1.3 x 105 daltons. The mixture of AsA or TR and Cu2+ fragmented calf thymus DNA to produce the fragmented DNA of which 5'-0H terminal groups have a mixture of free OH groups and phosphodiester linkage. The mixture of AsA or TR and Cu2+ was also found to fragment ADNA to produce dCMP predominantly as 5'-OH terminal nucleotides.

Original languageEnglish
Pages (from-to)671-678
Number of pages8
JournalJournal of Nutritional Science and Vitaminology
Volume29
Issue number6
DOIs
Publication statusPublished - 1983

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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