Abstract
<p>The induction of polyphenol oxidase (PPO) gene mutation through ion beam irradiation of protoplasts was attempted for the production of lettuce plants with a low browning property as a cut vegetable. It was clarified that the optimum protoplast density was 0.63–0.75 × 10<sup>4</sup> mL<sup>–1</sup> for shoot regeneration from protoplasts of lettuce 'Round'. Effects of <sup>12</sup>C<sup>6+</sup> ion beam irradiation on the growth of protoplasts were investigated, and the percentage of colony formation decreased as the irradiation dose increased. The dose leading to 50% cell growth as compared with the untreated control was approximately 3.3 Gy. Two out of 869 calli irradiated with <sup>12</sup>C<sup>6+</sup> ion beams were found to be <i>PPO</i> gene mutations by the TILLING method. PPO activities of the regenerated plants from the two mutant calli named 2Gy-3-36 and 5Gy-2-27 were preliminarily assayed, and lettuce plants from 2Gy-3-36 exhibited half of the PPO activity as compared with the non-irradiated controls. A part of the M<sub>2</sub> population from 2Gy-2-36 exhibited the property of no band of heteroduplex cleavage by <i>Cel</i> I, with markedly low PPO activity as the control.</p>
| Translated title of the contribution | Low Polyphenol Oxidase Mutant Induced by <sup>12</sup>C<sup>6+</sup> Ion Beam Irradiation to Protoplasts of Lettuce (<i>Lactuca sativa</i> L.) |
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| Original language | Japanese |
| Pages (from-to) | 347-353 |
| Number of pages | 7 |
| Journal | 園芸学研究 |
| Volume | 15 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2016 |