1-Hexylcarbamoyl-5-fluorouracil alters the expression of heat shock protein in HeLa cells.

T. Kusumoto, Y. Maehara, Y. Sakaguchi, Y. Emi, S. Kohnoe, K. Sugimachi

Research output: Contribution to journalArticle

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Abstract

We determined the effect of 1-hexylcarbamoyl-5-fluorouracil (HCFU), a masked compound of 5-fluorouracil, on the expression of heat shock protein (HSP) in heat-treated HeLa cells. We used a monoclonal anti-72-kDa heat shock protein antibody. At 37 degrees C, HSP was predominant in the cytoplasm of cells and heating to 43 degrees C for 30 min increased the synthesis of HSP in the nucleus. When HeLa cells were treated with heat and HCFU, at a concentration which showed evidence of synergy, nuclear staining of the cells decreased. Thus, the altered expression of HSP by HCFU is related to a synergism between heat and HCFU.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalAnti-cancer drugs
Volume2
Issue number1
DOIs
Publication statusPublished - Feb 1991

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Heat-Shock Proteins
HeLa Cells
Hot Temperature
HSP72 Heat-Shock Proteins
Fluorouracil
Heating
Cytoplasm
Staining and Labeling
1-hexylcarbamoyl-5-fluorouracil
Antibodies

All Science Journal Classification (ASJC) codes

  • Oncology
  • Pharmacology
  • Pharmacology (medical)
  • Cancer Research

Cite this

1-Hexylcarbamoyl-5-fluorouracil alters the expression of heat shock protein in HeLa cells. / Kusumoto, T.; Maehara, Y.; Sakaguchi, Y.; Emi, Y.; Kohnoe, S.; Sugimachi, K.

In: Anti-cancer drugs, Vol. 2, No. 1, 02.1991, p. 45-48.

Research output: Contribution to journalArticle

Kusumoto, T, Maehara, Y, Sakaguchi, Y, Emi, Y, Kohnoe, S & Sugimachi, K 1991, '1-Hexylcarbamoyl-5-fluorouracil alters the expression of heat shock protein in HeLa cells.', Anti-cancer drugs, vol. 2, no. 1, pp. 45-48. https://doi.org/10.1097/00001813-199102000-00006
Kusumoto, T. ; Maehara, Y. ; Sakaguchi, Y. ; Emi, Y. ; Kohnoe, S. ; Sugimachi, K. / 1-Hexylcarbamoyl-5-fluorouracil alters the expression of heat shock protein in HeLa cells. In: Anti-cancer drugs. 1991 ; Vol. 2, No. 1. pp. 45-48.
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