1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells

E. Hasegawa, H. Asagami, Dongchon Kang, S. Minakami, K. Takeshige

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

When rat pheochromocytoma PC12 cells are cultured with 1 mM 1-methyl-4-phenylpyridinium (MPP+), the number of viable cells decreases to one third in 4 days while the number increases ten-fold without MPP+. Oxygen consumption by mitochondria in the presence of malate is inhibited about 80% by the treatment of the cells with MPP+ for 4 days. Unexpectedly, succinate-dependent oxygen consumption is also inhibited to essentially the same extent as malate-dependent one. These results suggest that the impairment of the respiration mediated by succinate as well as malate is important as a mechanism of MPP+-induced cell death.

Original languageEnglish
Pages (from-to)409-413
Number of pages5
JournalBiochemistry and Molecular Biology International
Volume35
Issue number2
Publication statusPublished - 1995

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1-Methyl-4-phenylpyridinium
PC12 Cells
Succinic Acid
Pheochromocytoma
Oxygen Consumption
Rats
Oxygen
Mitochondria
Cell death
Respiration
Cell Death
Cell Count
Cells
malic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

1-methyl-4-phenylpyridinium (MPP+) inhibits mitochondrial oxygen consumption mediated by succinate as well as malate in rat pheochromocytoma PC12 cells. / Hasegawa, E.; Asagami, H.; Kang, Dongchon; Minakami, S.; Takeshige, K.

In: Biochemistry and Molecular Biology International, Vol. 35, No. 2, 1995, p. 409-413.

Research output: Contribution to journalArticle

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AU - Minakami, S.

AU - Takeshige, K.

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