1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of binary mixtures of antioxidants

Hiroshi Matsufuji, Ryoichiro Sasa, Yuki Honma, Hiromi Miyajima, Makoto Chino, Takeshi Yamazaki, Tomoko Shimamura, Hiroyuki Ukeda, Toshiro Matsui, Kiyoshi Matsumoto, Kazuo Yamagata

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Abstract

The effect of a binary mixture of antioxidants on the l,l-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity was investigated using 11 antioxidants as food additives or 24 natural antioxidants. Among 55 combinations of binary mixtures, including 11 antioxidants, BHA, BHT, catechin, quercetin, sesamol, ferulic acid, gallic acid, morin, ellagic acid, α-tocopherol, and δ-tocopherol, the DPPH radical scavenging activity of 36 combinations were significantly greater than the expected activity of individual antioxidants, resulting in synergistic effects. Also, one combination showed antagonistic effect. Among 276 combinations of 24 natural antioxidants, including 4 benzoic acids, 3 cinnamic acids, 12 flavonoids, 2 vitamins, and 3 diterpenes, 74 showed synergistic effects and 61 showed antagonistic effects. However, the ratios of the actual to expected activity of many synergistic and antagonistic combinations were about 1.1- 1.2 and 0.8-0.9, respectively.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalNippon Shokuhin Kagaku Kogaku Kaishi
Volume56
Issue number3
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Food Science

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    Matsufuji, H., Sasa, R., Honma, Y., Miyajima, H., Chino, M., Yamazaki, T., Shimamura, T., Ukeda, H., Matsui, T., Matsumoto, K., & Yamagata, K. (2009). 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of binary mixtures of antioxidants. Nippon Shokuhin Kagaku Kogaku Kaishi, 56(3), 129-136. https://doi.org/10.3136/nskkk.56.129