1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum

Yoshiaki Iizuka, Shigeru Ueda, Toshiro Hanada, Wataru Tani, Hiroshi Adachi, Riichi Haga, Mari Yamaguchi, Wataru Kurotani, Mitsuo Sekiguchi, Dongchon Kang, Shin Ichi Sakasegawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: There is a need for a pancreatic lipase (LIP) reference assay to provide an accurate base to which routine methods can be traceable. Methods: This study developed a novel LIP assay method in which 1,2-dioleoylglycerol (DODG) is the substrate and LIP activity is measured in a coupled enzymatic reaction from the increase in absorbance at 340 nm with production of NADPH. Results: With this method, LIP activity was linear up to 440 U/L (8-times expected upper limit of physiological concentration). When assayed manually, the between-laboratory variation for six samples surveyed at five laboratories was 3.8026.4 (CV) for samples containing about 20290 U/L LIP activity; when assayed using an automated analyzer, the range was 1.864.86 (four laboratories). Interference by >5 mmol/L glycerol and low specificity with post-heparin samples were noted, but in practice these are avoidable. Precision analyzed by automated assay of 49 samples twice in random order produced a covariance of 2.27 U/L, which is comparable to routine methods, and good correlations were obtained with five routine methods. Conclusions: Although further studies are required, the DODG method may be likely applicable as one candidate reference method.

Original languageEnglish
Pages (from-to)475-481
Number of pages7
JournalClinical Chemistry and Laboratory Medicine
Volume50
Issue number3
DOIs
Publication statusPublished - Mar 1 2012

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Lipase
Catalyst activity
Assays
Serum
NADP
Glycerol
Heparin
diolein
Substrates

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Iizuka, Y., Ueda, S., Hanada, T., Tani, W., Adachi, H., Haga, R., ... Sakasegawa, S. I. (2012). 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum. Clinical Chemistry and Laboratory Medicine, 50(3), 475-481. https://doi.org/10.1515/cclm.2011.811

1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum. / Iizuka, Yoshiaki; Ueda, Shigeru; Hanada, Toshiro; Tani, Wataru; Adachi, Hiroshi; Haga, Riichi; Yamaguchi, Mari; Kurotani, Wataru; Sekiguchi, Mitsuo; Kang, Dongchon; Sakasegawa, Shin Ichi.

In: Clinical Chemistry and Laboratory Medicine, Vol. 50, No. 3, 01.03.2012, p. 475-481.

Research output: Contribution to journalArticle

Iizuka, Y, Ueda, S, Hanada, T, Tani, W, Adachi, H, Haga, R, Yamaguchi, M, Kurotani, W, Sekiguchi, M, Kang, D & Sakasegawa, SI 2012, '1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum', Clinical Chemistry and Laboratory Medicine, vol. 50, no. 3, pp. 475-481. https://doi.org/10.1515/cclm.2011.811
Iizuka, Yoshiaki ; Ueda, Shigeru ; Hanada, Toshiro ; Tani, Wataru ; Adachi, Hiroshi ; Haga, Riichi ; Yamaguchi, Mari ; Kurotani, Wataru ; Sekiguchi, Mitsuo ; Kang, Dongchon ; Sakasegawa, Shin Ichi. / 1,2-Dioleoylglycerol method for pancreatic lipase catalytic activity in serum. In: Clinical Chemistry and Laboratory Medicine. 2012 ; Vol. 50, No. 3. pp. 475-481.
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