TY - JOUR
T1 - 1.5 Kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro
AU - Maehara, Y.
AU - Fujiyoshi, T.
AU - Takahashi, K.
AU - Yamamoto, M.
AU - Endo, H.
N1 - Funding Information:
We thank Dr. Tak. W. Mak for the generous supply of oncogene probes, M. Ohara for comments on the manuscript and Mr. N. Kinoshita and Miss M. Honda for assistance. This work was supported in part the research grants from the Ministry of Education, Science and Culture of Japan, and from the Japan Tobacco Industry.
PY - 1985/9/16
Y1 - 1985/9/16
N2 - A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3′ portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.
AB - A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3′ portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.
UR - http://www.scopus.com/inward/record.url?scp=0022401902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022401902&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(85)91310-5
DO - 10.1016/0006-291X(85)91310-5
M3 - Article
C2 - 2413848
AN - SCOPUS:0022401902
SN - 0006-291X
VL - 131
SP - 800
EP - 805
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -