1.5 Kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro

Y. Maehara, T. Fujiyoshi, K. Takahashi, M. Yamamoto, H. Endo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3′ portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.

Original languageEnglish
Pages (from-to)800-805
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume131
Issue number2
DOIs
Publication statusPublished - Sep 16 1985

Fingerprint

Rats
Tumors
Liver
Messenger RNA
Nucleotides
Clone Cells
Untranslated Regions
Neoplasms
Proteins
Protein Sorting Signals
Electrophoresis
RNA
Ascites
Open Reading Frames
Sequence Analysis
Hepatocellular Carcinoma
Complementary DNA
Cells
Amino Acids
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

1.5 Kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro. / Maehara, Y.; Fujiyoshi, T.; Takahashi, K.; Yamamoto, M.; Endo, H.

In: Biochemical and Biophysical Research Communications, Vol. 131, No. 2, 16.09.1985, p. 800-805.

Research output: Contribution to journalArticle

Maehara, Y. ; Fujiyoshi, T. ; Takahashi, K. ; Yamamoto, M. ; Endo, H. / 1.5 Kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro. In: Biochemical and Biophysical Research Communications. 1985 ; Vol. 131, No. 2. pp. 800-805.
@article{3471970e9b2e4467b32a1ab165779be3,
title = "1.5 Kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro",
abstract = "A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3′ portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.",
author = "Y. Maehara and T. Fujiyoshi and K. Takahashi and M. Yamamoto and H. Endo",
year = "1985",
month = "9",
day = "16",
doi = "10.1016/0006-291X(85)91310-5",
language = "English",
volume = "131",
pages = "800--805",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - 1.5 Kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro

AU - Maehara, Y.

AU - Fujiyoshi, T.

AU - Takahashi, K.

AU - Yamamoto, M.

AU - Endo, H.

PY - 1985/9/16

Y1 - 1985/9/16

N2 - A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3′ portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.

AB - A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3′ portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.

UR - http://www.scopus.com/inward/record.url?scp=0022401902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022401902&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(85)91310-5

DO - 10.1016/0006-291X(85)91310-5

M3 - Article

C2 - 2413848

AN - SCOPUS:0022401902

VL - 131

SP - 800

EP - 805

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -