2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides

Koji Yamamoto, Kenji Hamase, Kiyoshi Zaitsu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalJournal of Chromatography A
Volume1004
Issue number1-2
DOIs
Publication statusPublished - Jul 4 2003

Fingerprint

Monosaccharides
Fluorescence
Fetuins
Labeling
Calibration
Glycoproteins
Lasers
High Pressure Liquid Chromatography
Injections
2-amino-3-phenylpyrazine
Serum

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides. / Yamamoto, Koji; Hamase, Kenji; Zaitsu, Kiyoshi.

In: Journal of Chromatography A, Vol. 1004, No. 1-2, 04.07.2003, p. 99-106.

Research output: Contribution to journalArticle

@article{c1e3a464368047e292bcac81a93352b9,
title = "2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides",
abstract = "2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86{\%} (RSD) and 3.37-4.56{\%} (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.",
author = "Koji Yamamoto and Kenji Hamase and Kiyoshi Zaitsu",
year = "2003",
month = "7",
day = "4",
doi = "10.1016/S0021-9673(03)00452-7",
language = "English",
volume = "1004",
pages = "99--106",
journal = "Journal of Chromatography A",
issn = "0021-9673",
number = "1-2",

}

TY - JOUR

T1 - 2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides

AU - Yamamoto, Koji

AU - Hamase, Kenji

AU - Zaitsu, Kiyoshi

PY - 2003/7/4

Y1 - 2003/7/4

N2 - 2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.

AB - 2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.

UR - http://www.scopus.com/inward/record.url?scp=0038644223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038644223&partnerID=8YFLogxK

U2 - 10.1016/S0021-9673(03)00452-7

DO - 10.1016/S0021-9673(03)00452-7

M3 - Article

C2 - 12929966

AN - SCOPUS:0038644223

VL - 1004

SP - 99

EP - 106

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -