2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides

Koji Yamamoto, Kenji Hamase, Kiyoshi Zaitsu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalJournal of Chromatography A
Volume1004
Issue number1-2
DOIs
Publication statusPublished - Jul 4 2003

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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