Abstract
2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.
| Original language | English |
|---|---|
| Pages (from-to) | 99-106 |
| Number of pages | 8 |
| Journal | Journal of Chromatography A |
| Volume | 1004 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jul 4 2003 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Cite this
2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides. / Yamamoto, Koji; Hamase, Kenji; Zaitsu, Kiyoshi.
In: Journal of Chromatography A, Vol. 1004, No. 1-2, 04.07.2003, p. 99-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 2-Amino-3-phenylpyrazine, a sensitive fluorescence prelabeling reagent for the chromatographic or electrophoretic determination of saccharides
AU - Yamamoto, Koji
AU - Hamase, Kenji
AU - Zaitsu, Kiyoshi
PY - 2003/7/4
Y1 - 2003/7/4
N2 - 2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.
AB - 2-Amino-3-phenylpyrazine is found to be a sensitive fluorescence labeling reagent for saccharides with a reducing end. The labeled monosaccharides show strong fluorescence under various pH conditions, and could be analyzed by both HPLC and HPCE techniques. Laser induced fluorescence detection is also applicable. Following derivatization with 2-amino-3-phenylpyrazine, six monosaccharides are separated by an HPCE system within 23 min in the calibration range of 5 or 10 fmol to 5 pmol (injection amount). The within-day and day-to-day precisions of the monosaccharide determinations are 3.83-4.86% (RSD) and 3.37-4.56% (RSD), respectively. This method was successfully applied to the determination of component monosaccharides in a glycoprotein, bovine serum fetuin.
UR - http://www.scopus.com/inward/record.url?scp=0038644223&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038644223&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(03)00452-7
DO - 10.1016/S0021-9673(03)00452-7
M3 - Article
C2 - 12929966
AN - SCOPUS:0038644223
VL - 1004
SP - 99
EP - 106
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -