2-Arachidonoyl glycerol sensitizes the pars distalis and enhances forskolin-stimulated prolactin secretion in Syrian hamsters

Shinobu Yasuo, Claudia Fischer, Joerg Bojunga, Masayuki Iigo, Horst Werner Korf

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

2-Arachidonoyl glycerol (2-AG) is a major endocannabinoid and an important regulator of neuroendocrine system. In Syrian hamster and human, we found that 2-AG is synthesized in the hypophysial pars tuberalis (PT), an interface between photoperiodic melatonin signals and neuroendocrine output pathways. The target of 2-AG produced in the PT is likely to be the pars distalis (PD). Here we demonstrate that 2-AG in combination with forskolin stimulated prolactin secretion from PD organ cultures of Syrian hamsters, whereas incubation with 2-AG alone had no effect. Forskolin-induced prolactin secretion was also significantly enhanced when cultured PD tissue was preincubated with 2-AG. The stimulatory effects of 2-AG on prolactin secretion were blocked by AM251, a selective CB1 antagonist, and were still observed in the presence of quinpirole, a D2-class dopamine receptor agonist. 2-AG also enhanced prolactin secretion in the presence of adenosine, while it had little effect when applied together with adenosine diphosphate (ADP) and adenosine triphosphate (ATP). Moreover, the effect of forskolin was mimicked by adenosine in a dose-dependent manner. In conclusion, our data suggest that 2-AG sensitizes the PD tissue to potentiate the stimulating effects of forskolin and adenosine on prolactin secretion and thus provide novel insight into the mode of action of 2-AG in the PD.

Original languageEnglish
Pages (from-to)337-342
Number of pages6
JournalChronobiology International
Volume31
Issue number3
DOIs
Publication statusPublished - Apr 1 2014

Fingerprint

Mesocricetus
Colforsin
Prolactin
Adenosine
2-arachidonylglycerol
Quinpirole
Endocannabinoids
Neurosecretory Systems
Organ Culture Techniques
Dopamine Agonists
Melatonin
Adenosine Diphosphate
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

2-Arachidonoyl glycerol sensitizes the pars distalis and enhances forskolin-stimulated prolactin secretion in Syrian hamsters. / Yasuo, Shinobu; Fischer, Claudia; Bojunga, Joerg; Iigo, Masayuki; Korf, Horst Werner.

In: Chronobiology International, Vol. 31, No. 3, 01.04.2014, p. 337-342.

Research output: Contribution to journalArticle

Yasuo, Shinobu ; Fischer, Claudia ; Bojunga, Joerg ; Iigo, Masayuki ; Korf, Horst Werner. / 2-Arachidonoyl glycerol sensitizes the pars distalis and enhances forskolin-stimulated prolactin secretion in Syrian hamsters. In: Chronobiology International. 2014 ; Vol. 31, No. 3. pp. 337-342.
@article{32eef618c2474afead8da546ddadfc88,
title = "2-Arachidonoyl glycerol sensitizes the pars distalis and enhances forskolin-stimulated prolactin secretion in Syrian hamsters",
abstract = "2-Arachidonoyl glycerol (2-AG) is a major endocannabinoid and an important regulator of neuroendocrine system. In Syrian hamster and human, we found that 2-AG is synthesized in the hypophysial pars tuberalis (PT), an interface between photoperiodic melatonin signals and neuroendocrine output pathways. The target of 2-AG produced in the PT is likely to be the pars distalis (PD). Here we demonstrate that 2-AG in combination with forskolin stimulated prolactin secretion from PD organ cultures of Syrian hamsters, whereas incubation with 2-AG alone had no effect. Forskolin-induced prolactin secretion was also significantly enhanced when cultured PD tissue was preincubated with 2-AG. The stimulatory effects of 2-AG on prolactin secretion were blocked by AM251, a selective CB1 antagonist, and were still observed in the presence of quinpirole, a D2-class dopamine receptor agonist. 2-AG also enhanced prolactin secretion in the presence of adenosine, while it had little effect when applied together with adenosine diphosphate (ADP) and adenosine triphosphate (ATP). Moreover, the effect of forskolin was mimicked by adenosine in a dose-dependent manner. In conclusion, our data suggest that 2-AG sensitizes the PD tissue to potentiate the stimulating effects of forskolin and adenosine on prolactin secretion and thus provide novel insight into the mode of action of 2-AG in the PD.",
author = "Shinobu Yasuo and Claudia Fischer and Joerg Bojunga and Masayuki Iigo and Korf, {Horst Werner}",
year = "2014",
month = "4",
day = "1",
doi = "10.3109/07420528.2013.852104",
language = "English",
volume = "31",
pages = "337--342",
journal = "Chronobiology International",
issn = "0742-0528",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - 2-Arachidonoyl glycerol sensitizes the pars distalis and enhances forskolin-stimulated prolactin secretion in Syrian hamsters

AU - Yasuo, Shinobu

AU - Fischer, Claudia

AU - Bojunga, Joerg

AU - Iigo, Masayuki

AU - Korf, Horst Werner

PY - 2014/4/1

Y1 - 2014/4/1

N2 - 2-Arachidonoyl glycerol (2-AG) is a major endocannabinoid and an important regulator of neuroendocrine system. In Syrian hamster and human, we found that 2-AG is synthesized in the hypophysial pars tuberalis (PT), an interface between photoperiodic melatonin signals and neuroendocrine output pathways. The target of 2-AG produced in the PT is likely to be the pars distalis (PD). Here we demonstrate that 2-AG in combination with forskolin stimulated prolactin secretion from PD organ cultures of Syrian hamsters, whereas incubation with 2-AG alone had no effect. Forskolin-induced prolactin secretion was also significantly enhanced when cultured PD tissue was preincubated with 2-AG. The stimulatory effects of 2-AG on prolactin secretion were blocked by AM251, a selective CB1 antagonist, and were still observed in the presence of quinpirole, a D2-class dopamine receptor agonist. 2-AG also enhanced prolactin secretion in the presence of adenosine, while it had little effect when applied together with adenosine diphosphate (ADP) and adenosine triphosphate (ATP). Moreover, the effect of forskolin was mimicked by adenosine in a dose-dependent manner. In conclusion, our data suggest that 2-AG sensitizes the PD tissue to potentiate the stimulating effects of forskolin and adenosine on prolactin secretion and thus provide novel insight into the mode of action of 2-AG in the PD.

AB - 2-Arachidonoyl glycerol (2-AG) is a major endocannabinoid and an important regulator of neuroendocrine system. In Syrian hamster and human, we found that 2-AG is synthesized in the hypophysial pars tuberalis (PT), an interface between photoperiodic melatonin signals and neuroendocrine output pathways. The target of 2-AG produced in the PT is likely to be the pars distalis (PD). Here we demonstrate that 2-AG in combination with forskolin stimulated prolactin secretion from PD organ cultures of Syrian hamsters, whereas incubation with 2-AG alone had no effect. Forskolin-induced prolactin secretion was also significantly enhanced when cultured PD tissue was preincubated with 2-AG. The stimulatory effects of 2-AG on prolactin secretion were blocked by AM251, a selective CB1 antagonist, and were still observed in the presence of quinpirole, a D2-class dopamine receptor agonist. 2-AG also enhanced prolactin secretion in the presence of adenosine, while it had little effect when applied together with adenosine diphosphate (ADP) and adenosine triphosphate (ATP). Moreover, the effect of forskolin was mimicked by adenosine in a dose-dependent manner. In conclusion, our data suggest that 2-AG sensitizes the PD tissue to potentiate the stimulating effects of forskolin and adenosine on prolactin secretion and thus provide novel insight into the mode of action of 2-AG in the PD.

UR - http://www.scopus.com/inward/record.url?scp=84897597228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897597228&partnerID=8YFLogxK

U2 - 10.3109/07420528.2013.852104

DO - 10.3109/07420528.2013.852104

M3 - Article

C2 - 24200164

AN - SCOPUS:84897597228

VL - 31

SP - 337

EP - 342

JO - Chronobiology International

JF - Chronobiology International

SN - 0742-0528

IS - 3

ER -