24-hour rhythm of aquaporin-3 function in the epidermis is regulated by molecular clocks

Naoya Matsunaga, Kazufumi Itcho, Kengo Hamamura, Eriko Ikeda, Hisako Ikeyama, Yoko Furuichi, Miyako Watanabe, Satoru Koyanagi, Shigehiro Ohdo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Aquaporin 3 (AQP3) is located in the basal layer of the epidermis and regulates biological functions of skin such as water content and trans-epidermal water loss. A recent study showed that the biological function of skin exhibits a 24-hour rhythm, but the molecular mechanism of the variation remains poorly understood. Here we show that mice mutated in the core clock component CLOCK (Clk/Clk) show decreased stratum corneum hydration. An extensive search for the underlying cause led us to identify AQP3 as a new regulator to control the 24-hour variation in biological functions of skin. In mouse epidermis of wild-type mice, mAqp3 exhibits circadian rhythms; however, these are significantly decreased in Clk/Clk. Luciferase reporter gene analysis revealed that transcription of mAqp3 is activated by D-site-binding protein, a clock gene. A human homolog, hAQP3, also exhibited significant oscillation in human keratinocyte (HaCaT) cells synchronized with medium containing 50% serum, and this rhythm was regulated by the endogenous CLOCK/BMAL1 heterodimer. These data indicate that although the molecular mechanisms underlying the rhythmic expression of mAqp3 and hAQP3 are different, clock genes are involved in time-dependent skin hydration. Our current findings provide a molecular link between the circadian clock and AQP3 function in mouse dorsal skin and HaCaT cells.

Original languageEnglish
Pages (from-to)1636-1644
Number of pages9
JournalJournal of Investigative Dermatology
Volume134
Issue number6
DOIs
Publication statusPublished - Jan 1 2014

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Aquaporin 3
Epidermis
Clocks
Skin
Genes
Hydration
Circadian Clocks
Water
Transcription
Circadian Rhythm
Luciferases
Keratinocytes
Reporter Genes
Cornea
Water content
Carrier Proteins
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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24-hour rhythm of aquaporin-3 function in the epidermis is regulated by molecular clocks. / Matsunaga, Naoya; Itcho, Kazufumi; Hamamura, Kengo; Ikeda, Eriko; Ikeyama, Hisako; Furuichi, Yoko; Watanabe, Miyako; Koyanagi, Satoru; Ohdo, Shigehiro.

In: Journal of Investigative Dermatology, Vol. 134, No. 6, 01.01.2014, p. 1636-1644.

Research output: Contribution to journalArticle

Matsunaga, Naoya ; Itcho, Kazufumi ; Hamamura, Kengo ; Ikeda, Eriko ; Ikeyama, Hisako ; Furuichi, Yoko ; Watanabe, Miyako ; Koyanagi, Satoru ; Ohdo, Shigehiro. / 24-hour rhythm of aquaporin-3 function in the epidermis is regulated by molecular clocks. In: Journal of Investigative Dermatology. 2014 ; Vol. 134, No. 6. pp. 1636-1644.
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