2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels.

Thasaneeya Kuboki, Fahsai Kantawong, Richard Burchmore, Matthew J. Dalby, Satoru Kidoaki

Research output: Contribution to journalArticle

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Abstract

The present study focuses on mechanotransduction in mesenchymal stem cells (MSCs) in response to matrix elasticity. By using photocurable gelatinous gels with tunable stiffness, proteomic profiles of MSCs cultured on tissue culture plastic, soft (3 kPa) and stiff (52 kPa) matrices were deciphered using 2-dimensional differential in-gel analysis (2D-DIGE). The DIGE data, tied to immunofluorescence, indicated abundance and organization changes in the cytoskeletonal proteins as well as differential regulation of important signaling-related proteins, stress-responsing proteins and also proteins involved in collagen synthesis. The major CSK proteins including actin, tubulin and vimentin of the cells cultured on the gels were remarkably changed their expressions. Significant down-regulation of α-tubulin and β-actin can be observed on gel samples in comparison to the rigid tissue culture plates. The expression abundance of vimentin appeared to be highest in the MSCs cultured on hard gels. These results suggested that the substrate stiffness significantly affects expression balances in cytoskeletal proteins of MSCs with some implications to cellular tensegrity.

Original languageEnglish
Pages (from-to)127-139
Number of pages13
JournalCell structure and function
Volume37
Issue number2
Publication statusPublished - 2012

Fingerprint

Hydrogels
Elasticity
Mesenchymal Stromal Cells
Proteomics
Gels
Vimentin
Tubulin
Actins
Proteins
Cytoskeletal Proteins
Heat-Shock Proteins
Plastics
Fluorescent Antibody Technique
Cultured Cells
Collagen
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels. / Kuboki, Thasaneeya; Kantawong, Fahsai; Burchmore, Richard; Dalby, Matthew J.; Kidoaki, Satoru.

In: Cell structure and function, Vol. 37, No. 2, 2012, p. 127-139.

Research output: Contribution to journalArticle

Kuboki, Thasaneeya ; Kantawong, Fahsai ; Burchmore, Richard ; Dalby, Matthew J. ; Kidoaki, Satoru. / 2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels. In: Cell structure and function. 2012 ; Vol. 37, No. 2. pp. 127-139.
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