3-Methyladenine specifically inhibits retrograde transport of cation-independent mannose 6-phosphate/insulin-like growth factor II receptor from the early endosome to the TGN

Kaori Hirosako, Hiroshi Imasato, Yuko Hirota, Toshio Kuronita, Naoko Masuyama, Misa Nishioka, Atsushi Umeda, Hideaki Fujita, Masaru Himeno, Yoshitaka Tanaka

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

3-Methyladenine (3-MA), a well-known inhibitor of autophagic sequestration, can also prevent class III phosphatidylinositide (PI) 3-kinase activity, which is required for many processes in endosomal membrane trafficking. Although much is known about the effects of other PI 3-kinase inhibitors, such as wortmannin and LY294002, on endosomal membrane trafficking, little is known about those of 3-MA. Here we show that the treatment of cells with 3-MA results in a specific redistribution of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor (MPR300) from the trans-Golgi network (TGN) to early/recycling endosomal compartments containing internalized transferrin. Importantly, in contrast to wortmannin and LY294002, 3-MA did not cause the enlargement of late endosomal/lysosomal compartments. The results suggest that the effect of 3-MA is restricted to the retrieval of MPR300 from early/ recycling endosomes.

Original languageEnglish
Pages (from-to)845-852
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number3
DOIs
Publication statusPublished - Apr 9 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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