Abstract
3-Methyladenine (3-MA), a well-known inhibitor of autophagic sequestration, can also prevent class III phosphatidylinositide (PI) 3-kinase activity, which is required for many processes in endosomal membrane trafficking. Although much is known about the effects of other PI 3-kinase inhibitors, such as wortmannin and LY294002, on endosomal membrane trafficking, little is known about those of 3-MA. Here we show that the treatment of cells with 3-MA results in a specific redistribution of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor (MPR300) from the trans-Golgi network (TGN) to early/recycling endosomal compartments containing internalized transferrin. Importantly, in contrast to wortmannin and LY294002, 3-MA did not cause the enlargement of late endosomal/lysosomal compartments. The results suggest that the effect of 3-MA is restricted to the retrieval of MPR300 from early/ recycling endosomes.
| Original language | English |
|---|---|
| Pages (from-to) | 845-852 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 316 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 9 2004 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
3-Methyladenine specifically inhibits retrograde transport of cation-independent mannose 6-phosphate/insulin-like growth factor II receptor from the early endosome to the TGN. / Hirosako, Kaori; Imasato, Hiroshi; Hirota, Yuko; Kuronita, Toshio; Masuyama, Naoko; Nishioka, Misa; Umeda, Atsushi; Fujita, Hideaki; Himeno, Masaru; Tanaka, Yoshitaka.
In: Biochemical and Biophysical Research Communications, Vol. 316, No. 3, 09.04.2004, p. 845-852.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 3-Methyladenine specifically inhibits retrograde transport of cation-independent mannose 6-phosphate/insulin-like growth factor II receptor from the early endosome to the TGN
AU - Hirosako, Kaori
AU - Imasato, Hiroshi
AU - Hirota, Yuko
AU - Kuronita, Toshio
AU - Masuyama, Naoko
AU - Nishioka, Misa
AU - Umeda, Atsushi
AU - Fujita, Hideaki
AU - Himeno, Masaru
AU - Tanaka, Yoshitaka
PY - 2004/4/9
Y1 - 2004/4/9
N2 - 3-Methyladenine (3-MA), a well-known inhibitor of autophagic sequestration, can also prevent class III phosphatidylinositide (PI) 3-kinase activity, which is required for many processes in endosomal membrane trafficking. Although much is known about the effects of other PI 3-kinase inhibitors, such as wortmannin and LY294002, on endosomal membrane trafficking, little is known about those of 3-MA. Here we show that the treatment of cells with 3-MA results in a specific redistribution of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor (MPR300) from the trans-Golgi network (TGN) to early/recycling endosomal compartments containing internalized transferrin. Importantly, in contrast to wortmannin and LY294002, 3-MA did not cause the enlargement of late endosomal/lysosomal compartments. The results suggest that the effect of 3-MA is restricted to the retrieval of MPR300 from early/ recycling endosomes.
AB - 3-Methyladenine (3-MA), a well-known inhibitor of autophagic sequestration, can also prevent class III phosphatidylinositide (PI) 3-kinase activity, which is required for many processes in endosomal membrane trafficking. Although much is known about the effects of other PI 3-kinase inhibitors, such as wortmannin and LY294002, on endosomal membrane trafficking, little is known about those of 3-MA. Here we show that the treatment of cells with 3-MA results in a specific redistribution of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor (MPR300) from the trans-Golgi network (TGN) to early/recycling endosomal compartments containing internalized transferrin. Importantly, in contrast to wortmannin and LY294002, 3-MA did not cause the enlargement of late endosomal/lysosomal compartments. The results suggest that the effect of 3-MA is restricted to the retrieval of MPR300 from early/ recycling endosomes.
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U2 - 10.1016/j.bbrc.2004.02.119
DO - 10.1016/j.bbrc.2004.02.119
M3 - Article
VL - 316
SP - 845
EP - 852
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -