TY - JOUR
T1 - 3-Methyladenine specifically inhibits retrograde transport of cation-independent mannose 6-phosphate/insulin-like growth factor II receptor from the early endosome to the TGN
AU - Hirosako, Kaori
AU - Imasato, Hiroshi
AU - Hirota, Yuko
AU - Kuronita, Toshio
AU - Masuyama, Naoko
AU - Nishioka, Misa
AU - Umeda, Atsushi
AU - Fujita, Hideaki
AU - Himeno, Masaru
AU - Tanaka, Yoshitaka
N1 - Funding Information:
We thank Drs. G. Kimura (Kyushu University, Japan) and K. Akasaki (Fukuyama University, Japan) for providing rat 3Y1-B cells and mouse monoclonal antibodies against LGP85, respectively. This research was supported in part by grants from the Ministry of Labor, Health and Welfare of Japan and the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2004/4/9
Y1 - 2004/4/9
N2 - 3-Methyladenine (3-MA), a well-known inhibitor of autophagic sequestration, can also prevent class III phosphatidylinositide (PI) 3-kinase activity, which is required for many processes in endosomal membrane trafficking. Although much is known about the effects of other PI 3-kinase inhibitors, such as wortmannin and LY294002, on endosomal membrane trafficking, little is known about those of 3-MA. Here we show that the treatment of cells with 3-MA results in a specific redistribution of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor (MPR300) from the trans-Golgi network (TGN) to early/recycling endosomal compartments containing internalized transferrin. Importantly, in contrast to wortmannin and LY294002, 3-MA did not cause the enlargement of late endosomal/lysosomal compartments. The results suggest that the effect of 3-MA is restricted to the retrieval of MPR300 from early/ recycling endosomes.
AB - 3-Methyladenine (3-MA), a well-known inhibitor of autophagic sequestration, can also prevent class III phosphatidylinositide (PI) 3-kinase activity, which is required for many processes in endosomal membrane trafficking. Although much is known about the effects of other PI 3-kinase inhibitors, such as wortmannin and LY294002, on endosomal membrane trafficking, little is known about those of 3-MA. Here we show that the treatment of cells with 3-MA results in a specific redistribution of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor (MPR300) from the trans-Golgi network (TGN) to early/recycling endosomal compartments containing internalized transferrin. Importantly, in contrast to wortmannin and LY294002, 3-MA did not cause the enlargement of late endosomal/lysosomal compartments. The results suggest that the effect of 3-MA is restricted to the retrieval of MPR300 from early/ recycling endosomes.
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U2 - 10.1016/j.bbrc.2004.02.119
DO - 10.1016/j.bbrc.2004.02.119
M3 - Article
C2 - 15033478
AN - SCOPUS:12144291442
VL - 316
SP - 845
EP - 852
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -