TY - JOUR
T1 - 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation
AU - Re, Federica
AU - Sartore, Luciana
AU - Moulisova, Vladimira
AU - Cantini, Marco
AU - Almici, Camillo
AU - Bianchetti, Andrea
AU - Chinello, Clizia
AU - Dey, Kamol
AU - Agnelli, Silvia
AU - Manferdini, Cristina
AU - Bernardi, Simona
AU - Lopomo, Nicola F.
AU - Sardini, Emilio
AU - Borsani, Elisa
AU - Rodella, Luigi F.
AU - Savoldi, Fabio
AU - Paganelli, Corrado
AU - Guizzi, Pierangelo
AU - Lisignoli, Gina
AU - Magni, Fulvio
AU - Salmeron-Sanchez, Manuel
AU - Russo, Domenico
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The work was funded by Brescia University Health & Wealth Research Program, Fondazione Comunità Bresciana, European Research Council (ERC—HealInSynergy 306990), EPSRC (EP/ P001114/1) and MRC (MR/S005412/1). This work was also funded by a grant from the UK Regenerative Medicine Platform.
Funding Information:
The authors are highly grateful to the Brescia section of Associazione Donatrici Italiane Sangue Cordone Ombelicale (ADISCO) for supporting the research with hemopoietic and mesenchymal stem cells and Sterigenics Italy (Bologna, Italy) for sterilizing the materials. The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The work was funded by Brescia University Health & Wealth Research Program, Fondazione Comunità Bresciana, European Research Council (ERC—HealInSynergy 306990), EPSRC (EP/P001114/1) and MRC (MR/S005412/1). This work was also funded by a grant from the UK Regenerative Medicine Platform.
Publisher Copyright:
© The Author(s) 2019.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.
AB - Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.
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U2 - 10.1177/2041731419845852
DO - 10.1177/2041731419845852
M3 - Article
AN - SCOPUS:85065184450
SN - 2041-7314
VL - 10
JO - Journal of Tissue Engineering
JF - Journal of Tissue Engineering
ER -
