TY - GEN
T1 - 3D rotation of oocyte based on vibration-induced flow control integrated with label-free polarizing observation
AU - Hayakawa, Takeshi
AU - Sakuma, Shinya
AU - Arai, Fumihito
N1 - Funding Information:
This study was supported by JSPS Grant-in-Aid for Challenging Exploratory Research (No. 15K13909).
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - We present a novel 3D cell rotation method based on a vibration-induced flow. By applying circular vibration to a microchip having micropillar patterns, local flow is induced around each micropillar. Thus, we can induce the rotational flow when we pattern three micropillars in triangular configuration and the rotational flow can be utilized for rotational manipulation of cells. Additionally, we can achieve the rotation in vertical direction by applying circular vibration in vertical direction. Furthermore, we integrated polarizing microscopy to the rotation system for label-free observation of internal structure of oocytes. We succeeded in rotation of oocyte with 63.7 degrees/s and demonstrated polarizing observation of oocyte.
AB - We present a novel 3D cell rotation method based on a vibration-induced flow. By applying circular vibration to a microchip having micropillar patterns, local flow is induced around each micropillar. Thus, we can induce the rotational flow when we pattern three micropillars in triangular configuration and the rotational flow can be utilized for rotational manipulation of cells. Additionally, we can achieve the rotation in vertical direction by applying circular vibration in vertical direction. Furthermore, we integrated polarizing microscopy to the rotation system for label-free observation of internal structure of oocytes. We succeeded in rotation of oocyte with 63.7 degrees/s and demonstrated polarizing observation of oocyte.
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M3 - Conference contribution
AN - SCOPUS:84983297364
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 32
EP - 34
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -