Abstract
5α-Cholest-8(14)-en-3β-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to animals. Described herein are the results of experiments that indicate the presence of the 15-ketosterol in rat skin. The 15-ketosterol was, after purification by medium pressure liquid chromatography on Lichroprep RP-8 columns, thin-layer chromatography on silica gel G, and reverse phase high performance liquid chromatography, characterized by gas-liquid chromatography-mass spectrometry in the form of its trimethylsilyl ether derivative. The use of an internal standard containing both tritium and deuterium permitted the determination of the levels of the 15-ketosterol by mass fragmentography. The results of five separate analyses of portions of the skin of a male Sprague Dawley rat showed a mean value of 84.5 ± 4.1 (SEM) ng per g. Analyses of hair samples of ten male Sprague Dawley rats indicated a mean level of 143 ± 19 (SEM) ng per g of hair. Most (~ 72%) of the 15-ketosterol in hair was esterified. This report constitutes the first isolation of the 15-ketosterol from animal tissues.
| Original language | English |
|---|---|
| Pages (from-to) | 1039-1054 |
| Number of pages | 16 |
| Journal | Journal of Lipid Research |
| Volume | 29 |
| Issue number | 8 |
| Publication status | Published - Jan 1 1988 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Endocrinology
- Cell Biology
Cite this
5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism : Occurrence in rat skin. / Emmons, G. T.; Pyrek St., J.; Dam, R.; Martin, M.; Kudo, Keiko; Schroepfer, G. J.
In: Journal of Lipid Research, Vol. 29, No. 8, 01.01.1988, p. 1039-1054.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism
T2 - Occurrence in rat skin
AU - Emmons, G. T.
AU - Pyrek St., J.
AU - Dam, R.
AU - Martin, M.
AU - Kudo, Keiko
AU - Schroepfer, G. J.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - 5α-Cholest-8(14)-en-3β-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to animals. Described herein are the results of experiments that indicate the presence of the 15-ketosterol in rat skin. The 15-ketosterol was, after purification by medium pressure liquid chromatography on Lichroprep RP-8 columns, thin-layer chromatography on silica gel G, and reverse phase high performance liquid chromatography, characterized by gas-liquid chromatography-mass spectrometry in the form of its trimethylsilyl ether derivative. The use of an internal standard containing both tritium and deuterium permitted the determination of the levels of the 15-ketosterol by mass fragmentography. The results of five separate analyses of portions of the skin of a male Sprague Dawley rat showed a mean value of 84.5 ± 4.1 (SEM) ng per g. Analyses of hair samples of ten male Sprague Dawley rats indicated a mean level of 143 ± 19 (SEM) ng per g of hair. Most (~ 72%) of the 15-ketosterol in hair was esterified. This report constitutes the first isolation of the 15-ketosterol from animal tissues.
AB - 5α-Cholest-8(14)-en-3β-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to animals. Described herein are the results of experiments that indicate the presence of the 15-ketosterol in rat skin. The 15-ketosterol was, after purification by medium pressure liquid chromatography on Lichroprep RP-8 columns, thin-layer chromatography on silica gel G, and reverse phase high performance liquid chromatography, characterized by gas-liquid chromatography-mass spectrometry in the form of its trimethylsilyl ether derivative. The use of an internal standard containing both tritium and deuterium permitted the determination of the levels of the 15-ketosterol by mass fragmentography. The results of five separate analyses of portions of the skin of a male Sprague Dawley rat showed a mean value of 84.5 ± 4.1 (SEM) ng per g. Analyses of hair samples of ten male Sprague Dawley rats indicated a mean level of 143 ± 19 (SEM) ng per g of hair. Most (~ 72%) of the 15-ketosterol in hair was esterified. This report constitutes the first isolation of the 15-ketosterol from animal tissues.
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M3 - Article
C2 - 3183517
AN - SCOPUS:0023706095
VL - 29
SP - 1039
EP - 1054
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 8
ER -