5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism: Occurrence in rat skin

G. T. Emmons, J. Pyrek St., R. Dam, M. Martin, Keiko Kudo, G. J. Schroepfer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

5α-Cholest-8(14)-en-3β-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to animals. Described herein are the results of experiments that indicate the presence of the 15-ketosterol in rat skin. The 15-ketosterol was, after purification by medium pressure liquid chromatography on Lichroprep RP-8 columns, thin-layer chromatography on silica gel G, and reverse phase high performance liquid chromatography, characterized by gas-liquid chromatography-mass spectrometry in the form of its trimethylsilyl ether derivative. The use of an internal standard containing both tritium and deuterium permitted the determination of the levels of the 15-ketosterol by mass fragmentography. The results of five separate analyses of portions of the skin of a male Sprague Dawley rat showed a mean value of 84.5 ± 4.1 (SEM) ng per g. Analyses of hair samples of ten male Sprague Dawley rats indicated a mean level of 143 ± 19 (SEM) ng per g of hair. Most (~ 72%) of the 15-ketosterol in hair was esterified. This report constitutes the first isolation of the 15-ketosterol from animal tissues.

Original languageEnglish
Pages (from-to)1039-1054
Number of pages16
JournalJournal of Lipid Research
Volume29
Issue number8
Publication statusPublished - Jan 1 1988

Fingerprint

Metabolism
Hair
Rats
Skin
Cholesterol
Liquid chromatography
Sprague Dawley Rats
Animals
Anticholesteremic Agents
Thin layer chromatography
Scanning electron microscopy
Tritium
Deuterium
Biosynthesis
Silica Gel
High performance liquid chromatography
Reverse-Phase Chromatography
Thin Layer Chromatography
Liquid Chromatography
Gas chromatography

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Emmons, G. T., Pyrek St., J., Dam, R., Martin, M., Kudo, K., & Schroepfer, G. J. (1988). 5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism: Occurrence in rat skin. Journal of Lipid Research, 29(8), 1039-1054.

5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism : Occurrence in rat skin. / Emmons, G. T.; Pyrek St., J.; Dam, R.; Martin, M.; Kudo, Keiko; Schroepfer, G. J.

In: Journal of Lipid Research, Vol. 29, No. 8, 01.01.1988, p. 1039-1054.

Research output: Contribution to journalArticle

Emmons, GT, Pyrek St., J, Dam, R, Martin, M, Kudo, K & Schroepfer, GJ 1988, '5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism: Occurrence in rat skin', Journal of Lipid Research, vol. 29, no. 8, pp. 1039-1054.
Emmons GT, Pyrek St. J, Dam R, Martin M, Kudo K, Schroepfer GJ. 5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism: Occurrence in rat skin. Journal of Lipid Research. 1988 Jan 1;29(8):1039-1054.
Emmons, G. T. ; Pyrek St., J. ; Dam, R. ; Martin, M. ; Kudo, Keiko ; Schroepfer, G. J. / 5α-cholest-8(14)-en-3β-ol-15-one, a potent regulator of cholesterol metabolism : Occurrence in rat skin. In: Journal of Lipid Research. 1988 ; Vol. 29, No. 8. pp. 1039-1054.
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