800 fold signal enhancements by transient isotachophoresis for immunoassay of HSA on standard cross channel microchips

M. R. Mohamadi, L. Mahmoudian, N. Kaji, M. Tokeshi, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A fully automated preconcentration and stacking method based on transient isotachophoresis (ITP) on a standard cross channel microchip made of poly (methylmethacrylate) PMMA has been reported. Previously reported ITP has been either on a special chip design or required a hydrodynamic pressure for sample injection and a pause in the electrophoresis process which caused low reproducibility in analysis. In this work sample injection, preconcentration and separation are done continuously and are controlled solely by a sequential voltage switching program. The ITP followed by nondenaturing gel electrophoresis was applied for immunoassay of HSA.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1402-1404
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Externally publishedYes
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: Oct 7 2007Oct 11 2007

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period10/7/0710/11/07

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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