A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation

Ryo Goitsuka, Hideki Kanazashi, Hiroki Sasanuma, Yu ichi Fujimura, Yuri Hidaka, Akiko Tatsuno, Chisei Ra, Katsuhiko Hayashi, Daisuke Kitamura

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Cross-linking of the high-affinity IgE receptor (FcεRI) on mast cells by IgE-antigen complex triggers signal transduction cascades leading to the release of inflammatory mediators and production of cytokines, which are critical for the development of allergic reactions. We have identified a novel member of the BASH/SLP-76 immunoreceptor-coupled adaptor family expressed in mast cells, termed MIST (for mast cell immunoreceptor signal transducer), which has later been found to be identical to a recently reported cytokine-dependent hemopoietic cell linker, Clnk. Upon FcεRI cross-linking, MIST/Clnk is tyrosine phosphorylated and associates with signaling proteins, phospholipase Cγ, Vav, Grb2 and linker for activation of T cells (LAT). Overexpression of a mutant form of MIST/Clnk inhibited FcεRI-mediated degranulation, increase in intracellular Ca2+, NF-AT activation and phosphorylation of LAT. As a crucial signaling component for FcεRI-induced mast cell degranulation, MIST/Clnk might serve as a target for anti-allergic therapy.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalInternational immunology
Volume12
Issue number4
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation'. Together they form a unique fingerprint.

Cite this