A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation

Ryo Goitsuka, Hideki Kanazashi, Hiroki Sasanuma, Yu ichi Fujimura, Yuri Hidaka, Akiko Tatsuno, Chisei Ra, Katsuhiko Hayashi, Daisuke Kitamura

Research output: Contribution to journalArticle

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Abstract

Cross-linking of the high-affinity IgE receptor (FcεRI) on mast cells by IgE-antigen complex triggers signal transduction cascades leading to the release of inflammatory mediators and production of cytokines, which are critical for the development of allergic reactions. We have identified a novel member of the BASH/SLP-76 immunoreceptor-coupled adaptor family expressed in mast cells, termed MIST (for mast cell immunoreceptor signal transducer), which has later been found to be identical to a recently reported cytokine-dependent hemopoietic cell linker, Clnk. Upon FcεRI cross-linking, MIST/Clnk is tyrosine phosphorylated and associates with signaling proteins, phospholipase Cγ, Vav, Grb2 and linker for activation of T cells (LAT). Overexpression of a mutant form of MIST/Clnk inhibited FcεRI-mediated degranulation, increase in intracellular Ca2+, NF-AT activation and phosphorylation of LAT. As a crucial signaling component for FcεRI-induced mast cell degranulation, MIST/Clnk might serve as a target for anti-allergic therapy.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalInternational Immunology
Volume12
Issue number4
DOIs
Publication statusPublished - Jan 1 2000

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IgE Receptors
Cell Degranulation
Transducers
Mast Cells
Proteins
Cytokines
T-Lymphocytes
Anti-Allergic Agents
Type C Phospholipases
Protein C
Immunoglobulin E
Tyrosine
Signal Transduction
Hypersensitivity
Phosphorylation
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Goitsuka, R., Kanazashi, H., Sasanuma, H., Fujimura, Y. I., Hidaka, Y., Tatsuno, A., ... Kitamura, D. (2000). A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation. International Immunology, 12(4), 573-580. https://doi.org/10.1093/intimm/12.4.573

A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation. / Goitsuka, Ryo; Kanazashi, Hideki; Sasanuma, Hiroki; Fujimura, Yu ichi; Hidaka, Yuri; Tatsuno, Akiko; Ra, Chisei; Hayashi, Katsuhiko; Kitamura, Daisuke.

In: International Immunology, Vol. 12, No. 4, 01.01.2000, p. 573-580.

Research output: Contribution to journalArticle

Goitsuka, R, Kanazashi, H, Sasanuma, H, Fujimura, YI, Hidaka, Y, Tatsuno, A, Ra, C, Hayashi, K & Kitamura, D 2000, 'A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation', International Immunology, vol. 12, no. 4, pp. 573-580. https://doi.org/10.1093/intimm/12.4.573
Goitsuka, Ryo ; Kanazashi, Hideki ; Sasanuma, Hiroki ; Fujimura, Yu ichi ; Hidaka, Yuri ; Tatsuno, Akiko ; Ra, Chisei ; Hayashi, Katsuhiko ; Kitamura, Daisuke. / A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE receptor-mediated mast cell degranulation. In: International Immunology. 2000 ; Vol. 12, No. 4. pp. 573-580.
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