A bidirectional origin of replication maps to the major noncoding region of human mitochondrial DNA

Takehiro Yasukawa, Ming Yao Yang, Howard T. Jacobs, Ian J. Holt

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

In solid tissues of vertebrates, initiation of mitochondrial DNA replication encompasses a broad zone downstream of the major noncoding region (NCR). In contrast, analysis with two-dimensional agarose gel electrophoresis of mitochondrial DNA replication intermediates in cultured human cells revealed initiation concentrated in the NCR. Mapping of prominent free 5′ ends on the heavy strand of mitochondrial DNA identified two clusters of potential start sites. One mapped to the previously assigned origin of strand-asynchronous replication (OH); the other lay several hundred nucleotides away from OH, toward the other end of the NCR. The latter cluster is proposed to be the major site of bidirectional replication initiation on the basis of the following: its prominence is enhanced in cells amplifying mitochondrial DNA after experimentally induced mitochondrial DNA depletion; free 5′ ends are found in corresponding positions on the opposite strand; it is transient in nature; and it is associated with bubble arcs.

Original languageEnglish
Pages (from-to)651-662
Number of pages12
JournalMolecular Cell
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 10 2005
Externally publishedYes

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Replication Origin
Mitochondrial DNA
DNA Replication
Agar Gel Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Vertebrates
Cultured Cells
Nucleotides

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

A bidirectional origin of replication maps to the major noncoding region of human mitochondrial DNA. / Yasukawa, Takehiro; Yang, Ming Yao; Jacobs, Howard T.; Holt, Ian J.

In: Molecular Cell, Vol. 18, No. 6, 10.06.2005, p. 651-662.

Research output: Contribution to journalArticle

Yasukawa, Takehiro ; Yang, Ming Yao ; Jacobs, Howard T. ; Holt, Ian J. / A bidirectional origin of replication maps to the major noncoding region of human mitochondrial DNA. In: Molecular Cell. 2005 ; Vol. 18, No. 6. pp. 651-662.
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