TY - JOUR
T1 - A candidate factor that interacts with RF2, a restorer of fertility of Lead rice-type cytoplasmic male sterility in rice
AU - Fujii, Shinya
AU - Kazama, Tomohiko
AU - Ito, Yukihiro
AU - Kojima, Soichi
AU - Toriyama, Kinya
N1 - Funding Information:
This work was supported by MEXT/JSPS KAKENHI Grant Numbers 2338002, 24117502 and 26292002, and by Science and technology research promotion program for agriculture, forestry, fisheries and food industry (No. 26010A).
Publisher Copyright:
© 2014, Fujii et al.; licensee Springer.
PY - 2014
Y1 - 2014
N2 - Findings: To elucidate the protein that interacts with RF2, we performed yeast two-hybrid screening. We identified four genes and named RF2-interacting candidate factors (RIF1 to RIF4). A study of subcellular localization demonstrated that only RIF2 was targeted to mitochondria. A pull-down assay using E. coli-produced recombinant GST-tagged RF2 and His-tagged RIF2 confirmed that RF2 interacted with RIF2. RIF2 encodes ubiquitin domain-containing protein.Conclusions: These results suggest that RIF2 is a candidate factor of a fertility restoration complex of RF2.Background: The pollen function of cytoplasmic male sterile (CMS) plants is often recovered by the Restorer of fertility (Rf) gene encoded by the nuclear genome. An Rf gene of Lead rice type CMS, Rf2, encodes a small mitochondrial glycine-rich protein. RF2 is expected to function by interacting with other proteins, because RF2 has no motifs except for glycine-rich domain.
AB - Findings: To elucidate the protein that interacts with RF2, we performed yeast two-hybrid screening. We identified four genes and named RF2-interacting candidate factors (RIF1 to RIF4). A study of subcellular localization demonstrated that only RIF2 was targeted to mitochondria. A pull-down assay using E. coli-produced recombinant GST-tagged RF2 and His-tagged RIF2 confirmed that RF2 interacted with RIF2. RIF2 encodes ubiquitin domain-containing protein.Conclusions: These results suggest that RIF2 is a candidate factor of a fertility restoration complex of RF2.Background: The pollen function of cytoplasmic male sterile (CMS) plants is often recovered by the Restorer of fertility (Rf) gene encoded by the nuclear genome. An Rf gene of Lead rice type CMS, Rf2, encodes a small mitochondrial glycine-rich protein. RF2 is expected to function by interacting with other proteins, because RF2 has no motifs except for glycine-rich domain.
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U2 - 10.1186/s12284-014-0021-6
DO - 10.1186/s12284-014-0021-6
M3 - Article
AN - SCOPUS:84919743026
VL - 7
JO - Rice
JF - Rice
SN - 1939-8425
IS - 1
M1 - 21
ER -