The sonicate of human neutrophils converted leukotriene B4 to a polar product in aerobic condition in the presence of NADPH at a rate comparable to that of the intact cells. NADH could scarcely replace NADPH. The conversion was not observed in anaerobic conditions and was inhibited by carbon monoxide ( CO O2= 4 1) or by 1 mM p-chlormercuribenzoate, while it was not affected by 1 mM KCN, 5 mM NaN3, 200 μg/ml catalase, 100 mM mannitol, and 10 μg/ml superoxide dismutase. These observations suggest that the myeloperoxidase-H2O2-halide system and active oxygen species are not involved in the reaction. The activity was observed in the 100,000xg supernatant from the homogenate, in which cytochrome P-450 was not detected.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Dec 14 1984|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology