A cell-free preparation of human neutrophils catalyzing NADPH-dependent conversion of leukotriene B4

Hideki Sumimoto, Koichiro Takeshige, Hironori Sakai, Shigeki Minakami

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The sonicate of human neutrophils converted leukotriene B4 to a polar product in aerobic condition in the presence of NADPH at a rate comparable to that of the intact cells. NADH could scarcely replace NADPH. The conversion was not observed in anaerobic conditions and was inhibited by carbon monoxide ( CO O2= 4 1) or by 1 mM p-chlormercuribenzoate, while it was not affected by 1 mM KCN, 5 mM NaN3, 200 μg/ml catalase, 100 mM mannitol, and 10 μg/ml superoxide dismutase. These observations suggest that the myeloperoxidase-H2O2-halide system and active oxygen species are not involved in the reaction. The activity was observed in the 100,000xg supernatant from the homogenate, in which cytochrome P-450 was not detected.

Original languageEnglish
Pages (from-to)615-621
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume125
Issue number2
DOIs
Publication statusPublished - Dec 14 1984

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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