A Chemical Probe that Labels Human Pluripotent Stem Cells

Nao Hirata; Masato Nakagawa; Yuto Fujibayashi; Kaori Yamauchi; Asako Murata; Itsunari Minami; Maiko Tomioka; Takayuki Kondo; Ting Fang Kuo; Hiroshi Endo; Haruhisa Inoue; Shin Ichi Sato; Shin Ando; Yoshinori Kawazoe; Kazuhiro Aiba; Koh Nagata; Eihachiro Kawase; Young Tae Chang; Hirofumi Suemori; Koji Eto; Hiromitsu Nakauchi; Shinya Yamanaka; Norio Nakatsuji; Kazumitsu Ueda; Motonari Uesugi

doi:10.1016/j.celrep.2014.02.006

A Chemical Probe that Labels Human Pluripotent Stem Cells

Nao Hirata, Masato Nakagawa, Yuto Fujibayashi, Kaori Yamauchi, Asako Murata, Itsunari Minami, Maiko Tomioka, Takayuki Kondo, Ting Fang Kuo, Hiroshi Endo, Haruhisa Inoue, Shin Ichi Sato, Shin Ando, Yoshinori Kawazoe, Kazuhiro Aiba, Koh Nagata, Eihachiro Kawase, Young Tae Chang, Hirofumi Suemori, Koji EtoHiromitsu Nakauchi, Shinya Yamanaka, Norio Nakatsuji, Kazumitsu Ueda, Motonari Uesugi

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35 Citations (Scopus)

Abstract

A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.

Original language	English
Pages (from-to)	1165-1174
Number of pages	10
Journal	Cell Reports
Volume	6
Issue number	6
DOIs	https://doi.org/10.1016/j.celrep.2014.02.006
Publication status	Published - Mar 27 2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.celrep.2014.02.006

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Hirata, N., Nakagawa, M., Fujibayashi, Y., Yamauchi, K., Murata, A., Minami, I., Tomioka, M., Kondo, T., Kuo, T. F., Endo, H., Inoue, H., Sato, S. I., Ando, S., Kawazoe, Y., Aiba, K., Nagata, K., Kawase, E., Chang, Y. T., Suemori, H., ... Uesugi, M. (2014). A Chemical Probe that Labels Human Pluripotent Stem Cells. Cell Reports, 6(6), 1165-1174. https://doi.org/10.1016/j.celrep.2014.02.006

Hirata, N, Nakagawa, M, Fujibayashi, Y, Yamauchi, K, Murata, A, Minami, I, Tomioka, M, Kondo, T, Kuo, TF, Endo, H, Inoue, H, Sato, SI, Ando, S, Kawazoe, Y, Aiba, K, Nagata, K, Kawase, E, Chang, YT, Suemori, H, Eto, K, Nakauchi, H, Yamanaka, S, Nakatsuji, N, Ueda, K & Uesugi, M 2014, 'A Chemical Probe that Labels Human Pluripotent Stem Cells', Cell Reports, vol. 6, no. 6, pp. 1165-1174. https://doi.org/10.1016/j.celrep.2014.02.006

@article{6e79a4c2b6b043efb88ff27ade55778b,

title = "A Chemical Probe that Labels Human Pluripotent Stem Cells",

abstract = "A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.",

author = "Nao Hirata and Masato Nakagawa and Yuto Fujibayashi and Kaori Yamauchi and Asako Murata and Itsunari Minami and Maiko Tomioka and Takayuki Kondo and Kuo, {Ting Fang} and Hiroshi Endo and Haruhisa Inoue and Sato, {Shin Ichi} and Shin Ando and Yoshinori Kawazoe and Kazuhiro Aiba and Koh Nagata and Eihachiro Kawase and Chang, {Young Tae} and Hirofumi Suemori and Koji Eto and Hiromitsu Nakauchi and Shinya Yamanaka and Norio Nakatsuji and Kazumitsu Ueda and Motonari Uesugi",

note = "Funding Information: This work was supported in part by MEXT Leading Project, JSPS ( LR018 , 20228001 , 23710254 , 25221203 , and 21591079 ), the Bio-Oriented Technology Research Advancement Institution of Japan (BRAIN) , JST (CREST) , and JSPS Asian CORE Program Asian Chemical Biology Initiative. The M.U. research group participates in the Global COE program Integrated Materials Science (#B-09). iCeMS is supported by World Premier International Research Center Initiative (WPI), MEXT, Japan . The upgrade of the confocal microscope was supported by NEDO and Yokogawa Electric . S.Y. is a member without salary of the scientific advisory boards of iPierian, iPS Academia Japan, Megakaryon Corporation, and HEALIOS K.K. Japan. H.N. is a founder, shareholder, and scientific advisory board member of ReproCELL and Megakaryon. N.N. is a founder and shareholder of ReproCELL. ",

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doi = "10.1016/j.celrep.2014.02.006",

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T1 - A Chemical Probe that Labels Human Pluripotent Stem Cells

AU - Hirata, Nao

AU - Nakagawa, Masato

AU - Fujibayashi, Yuto

AU - Yamauchi, Kaori

AU - Murata, Asako

AU - Minami, Itsunari

AU - Tomioka, Maiko

AU - Kondo, Takayuki

AU - Kuo, Ting Fang

AU - Endo, Hiroshi

AU - Inoue, Haruhisa

AU - Sato, Shin Ichi

AU - Ando, Shin

AU - Kawazoe, Yoshinori

AU - Aiba, Kazuhiro

AU - Nagata, Koh

AU - Kawase, Eihachiro

AU - Chang, Young Tae

AU - Suemori, Hirofumi

AU - Eto, Koji

AU - Nakauchi, Hiromitsu

AU - Yamanaka, Shinya

AU - Nakatsuji, Norio

AU - Ueda, Kazumitsu

AU - Uesugi, Motonari

N1 - Funding Information: This work was supported in part by MEXT Leading Project, JSPS ( LR018 , 20228001 , 23710254 , 25221203 , and 21591079 ), the Bio-Oriented Technology Research Advancement Institution of Japan (BRAIN) , JST (CREST) , and JSPS Asian CORE Program Asian Chemical Biology Initiative. The M.U. research group participates in the Global COE program Integrated Materials Science (#B-09). iCeMS is supported by World Premier International Research Center Initiative (WPI), MEXT, Japan . The upgrade of the confocal microscope was supported by NEDO and Yokogawa Electric . S.Y. is a member without salary of the scientific advisory boards of iPierian, iPS Academia Japan, Megakaryon Corporation, and HEALIOS K.K. Japan. H.N. is a founder, shareholder, and scientific advisory board member of ReproCELL and Megakaryon. N.N. is a founder and shareholder of ReproCELL.

PY - 2014/3/27

Y1 - 2014/3/27

N2 - A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.

AB - A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.

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JF - Cell Reports

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ER -

A Chemical Probe that Labels Human Pluripotent Stem Cells

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