A Chinese hamster cDNA encoding a protein essential for phosphatidylserine synthase I activity

Osamu Kuge, Masahiro Nishijima, Yuzuru Akamatsu

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A phosphatidylserine-auxotrophic mutant of cultured Chinese hamster ovary (CHO) cells, PSA-3, is defective in phosphatidylserine synthase I activity. Transfection of PSA-3 cells with a cDNA expression library of CHO-K1 (the parent of PSA-3) yielded a phosphatidylserine-prototrophic transformant with normal phosphatidylserine synthase I activity. Using a cDNA segment retrieved from the transformant as a probe, a cDNA clone (pssA) responsible for phosphatidylserine prototrophy was isolated from the original cDNA library by colony filter hybridization. Introduction of the pssA cDNA into PSA-3 cells restored the phosphatidylserine content, and the resultant transformant exhibited 15-fold higher specific phosphatidylserine synthase I activity than CHO-K1 cells. The nucleotide sequence of the pssA cDNA contained a single long open reading frame capable of encoding a protein of 471 amino acid residues with several putative membrane-spanning domains. Our results indicated that the pssA cDNA encodes an integral membrane protein essential for phosphatidylserine synthase I activity.

Original languageEnglish
Pages (from-to)24184-24189
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number35
Publication statusPublished - Dec 15 1991
Externally publishedYes

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Cricetulus
Phosphatidylserines
Complementary DNA
Ovary
Proteins
Gene Library
Open Reading Frames
Transfection
Membrane Proteins
Clone Cells
phospholipid serine base exchange enzyme
Amino Acids
Nucleotides
Cells
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

A Chinese hamster cDNA encoding a protein essential for phosphatidylserine synthase I activity. / Kuge, Osamu; Nishijima, Masahiro; Akamatsu, Yuzuru.

In: Journal of Biological Chemistry, Vol. 266, No. 35, 15.12.1991, p. 24184-24189.

Research output: Contribution to journalArticle

Kuge, Osamu ; Nishijima, Masahiro ; Akamatsu, Yuzuru. / A Chinese hamster cDNA encoding a protein essential for phosphatidylserine synthase I activity. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 35. pp. 24184-24189.
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