A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci

Christopher T. Denny, Yasunobu Yoshikai, Tak W. Mak, Stephen D. Smith, Gregory F. Hollis, Ilan R. Kirsch

    Research output: Contribution to journalArticle

    90 Citations (Scopus)

    Abstract

    Specific chromosomal aberrations are associated with specific types of cancer (for review see ref. 1). The distinctiveness of each association has led to the belief that these chromosomal aberrations are clues to oncogenic events or to the state of differentiation in the malignant cell type2-6. Malignancies of T lymphocytes demonstrate such an association characterized most frequently by structural translocations or inversions of chromosomes 7 and 14 (refs 7-9). Analyses of these chromosomally marked tumours at the molecular level may therefore provide insight into the aetiology of the cancers as well as the mechanisms by which chromosomes break and rejoin. Here we report such an analysis of the tumour cell line SUP-T1 derived from a patient with childhood T-cell lymphoma carrying an inversion of one chromosome 14 between bands qll.2 and q32.3, that is10, inv(14) (qll.2; q32.2). These are the same chromosomal bands to which the T-cell receptor α-chain11,12 (14qll.2) and the immunoglobulin heavy-chain locus13 (14q32.3) have been assigned. Our analysis reveals that this morphological inversion of chromosome 14 was mediated by a site-specific recombination event between an immunoglobulin heavy-chain variable region (Ig VH) and a T-cell receptor (TCR) α-chain joining segment (TCR Jα). S 1 nuclease analysis shows that this hybrid gene is transcribed into poly(A)+ RNA.

    Original languageEnglish
    Pages (from-to)549-551
    Number of pages3
    JournalNature
    Volume320
    Issue number6062
    DOIs
    Publication statusPublished - Dec 1 1986

    Fingerprint

    Chromosomes, Human, Pair 14
    T-Cell Lymphoma
    T-Cell Antigen Receptor
    Genetic Recombination
    Immunoglobulins
    Immunoglobulin Heavy Chains
    Chromosome Aberrations
    Neoplasms
    Immunoglobulin Variable Region
    Chromosome Breakage
    Chromosomes, Human, Pair 7
    Tumor Cell Line
    T-Lymphocytes
    Messenger RNA
    Genes

    All Science Journal Classification (ASJC) codes

    • General

    Cite this

    Denny, C. T., Yoshikai, Y., Mak, T. W., Smith, S. D., Hollis, G. F., & Kirsch, I. R. (1986). A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci. Nature, 320(6062), 549-551. https://doi.org/10.1038/320549a0

    A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci. / Denny, Christopher T.; Yoshikai, Yasunobu; Mak, Tak W.; Smith, Stephen D.; Hollis, Gregory F.; Kirsch, Ilan R.

    In: Nature, Vol. 320, No. 6062, 01.12.1986, p. 549-551.

    Research output: Contribution to journalArticle

    Denny, Christopher T. ; Yoshikai, Yasunobu ; Mak, Tak W. ; Smith, Stephen D. ; Hollis, Gregory F. ; Kirsch, Ilan R. / A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci. In: Nature. 1986 ; Vol. 320, No. 6062. pp. 549-551.
    @article{38a15416402344e18801c1fb938bcd58,
    title = "A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci",
    abstract = "Specific chromosomal aberrations are associated with specific types of cancer (for review see ref. 1). The distinctiveness of each association has led to the belief that these chromosomal aberrations are clues to oncogenic events or to the state of differentiation in the malignant cell type2-6. Malignancies of T lymphocytes demonstrate such an association characterized most frequently by structural translocations or inversions of chromosomes 7 and 14 (refs 7-9). Analyses of these chromosomally marked tumours at the molecular level may therefore provide insight into the aetiology of the cancers as well as the mechanisms by which chromosomes break and rejoin. Here we report such an analysis of the tumour cell line SUP-T1 derived from a patient with childhood T-cell lymphoma carrying an inversion of one chromosome 14 between bands qll.2 and q32.3, that is10, inv(14) (qll.2; q32.2). These are the same chromosomal bands to which the T-cell receptor α-chain11,12 (14qll.2) and the immunoglobulin heavy-chain locus13 (14q32.3) have been assigned. Our analysis reveals that this morphological inversion of chromosome 14 was mediated by a site-specific recombination event between an immunoglobulin heavy-chain variable region (Ig VH) and a T-cell receptor (TCR) α-chain joining segment (TCR Jα). S 1 nuclease analysis shows that this hybrid gene is transcribed into poly(A)+ RNA.",
    author = "Denny, {Christopher T.} and Yasunobu Yoshikai and Mak, {Tak W.} and Smith, {Stephen D.} and Hollis, {Gregory F.} and Kirsch, {Ilan R.}",
    year = "1986",
    month = "12",
    day = "1",
    doi = "10.1038/320549a0",
    language = "English",
    volume = "320",
    pages = "549--551",
    journal = "Nature",
    issn = "0028-0836",
    publisher = "Nature Publishing Group",
    number = "6062",

    }

    TY - JOUR

    T1 - A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci

    AU - Denny, Christopher T.

    AU - Yoshikai, Yasunobu

    AU - Mak, Tak W.

    AU - Smith, Stephen D.

    AU - Hollis, Gregory F.

    AU - Kirsch, Ilan R.

    PY - 1986/12/1

    Y1 - 1986/12/1

    N2 - Specific chromosomal aberrations are associated with specific types of cancer (for review see ref. 1). The distinctiveness of each association has led to the belief that these chromosomal aberrations are clues to oncogenic events or to the state of differentiation in the malignant cell type2-6. Malignancies of T lymphocytes demonstrate such an association characterized most frequently by structural translocations or inversions of chromosomes 7 and 14 (refs 7-9). Analyses of these chromosomally marked tumours at the molecular level may therefore provide insight into the aetiology of the cancers as well as the mechanisms by which chromosomes break and rejoin. Here we report such an analysis of the tumour cell line SUP-T1 derived from a patient with childhood T-cell lymphoma carrying an inversion of one chromosome 14 between bands qll.2 and q32.3, that is10, inv(14) (qll.2; q32.2). These are the same chromosomal bands to which the T-cell receptor α-chain11,12 (14qll.2) and the immunoglobulin heavy-chain locus13 (14q32.3) have been assigned. Our analysis reveals that this morphological inversion of chromosome 14 was mediated by a site-specific recombination event between an immunoglobulin heavy-chain variable region (Ig VH) and a T-cell receptor (TCR) α-chain joining segment (TCR Jα). S 1 nuclease analysis shows that this hybrid gene is transcribed into poly(A)+ RNA.

    AB - Specific chromosomal aberrations are associated with specific types of cancer (for review see ref. 1). The distinctiveness of each association has led to the belief that these chromosomal aberrations are clues to oncogenic events or to the state of differentiation in the malignant cell type2-6. Malignancies of T lymphocytes demonstrate such an association characterized most frequently by structural translocations or inversions of chromosomes 7 and 14 (refs 7-9). Analyses of these chromosomally marked tumours at the molecular level may therefore provide insight into the aetiology of the cancers as well as the mechanisms by which chromosomes break and rejoin. Here we report such an analysis of the tumour cell line SUP-T1 derived from a patient with childhood T-cell lymphoma carrying an inversion of one chromosome 14 between bands qll.2 and q32.3, that is10, inv(14) (qll.2; q32.2). These are the same chromosomal bands to which the T-cell receptor α-chain11,12 (14qll.2) and the immunoglobulin heavy-chain locus13 (14q32.3) have been assigned. Our analysis reveals that this morphological inversion of chromosome 14 was mediated by a site-specific recombination event between an immunoglobulin heavy-chain variable region (Ig VH) and a T-cell receptor (TCR) α-chain joining segment (TCR Jα). S 1 nuclease analysis shows that this hybrid gene is transcribed into poly(A)+ RNA.

    UR - http://www.scopus.com/inward/record.url?scp=0022518317&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0022518317&partnerID=8YFLogxK

    U2 - 10.1038/320549a0

    DO - 10.1038/320549a0

    M3 - Article

    C2 - 3008004

    AN - SCOPUS:0022518317

    VL - 320

    SP - 549

    EP - 551

    JO - Nature

    JF - Nature

    SN - 0028-0836

    IS - 6062

    ER -