A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods

Hirotaro Kitazaki, Takeshi Mori, Jeong Hun Kang, Takuro Niidome, Masaharu Murata, Makoto Hashizume, Yoshiki Katayama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We succeeded in applying gold nanorods (NRs) for the detection of protein kinase (PK) activity based on a substrate-induced coagulation mechanism. Because NRs have cationic surface charges due to surface-adsorbed cetyltrimethylammonium bromide, phosphorylated substrate peptides, which gain augmented anionic charges by phosphorylation, induce coagulation of the NRs, resulting in a red-to-blue color change of the corresponding NR dispersion. A large absorption coefficient of the NRs at the near-IR region enabled clear-cut detection of the peptide-induced coagulation. IC 50 values of several inhibitors of c-Src determined by the present assay are consistent with reported values.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume99
DOIs
Publication statusPublished - Nov 1 2012

Fingerprint

Nanotubes
coagulation
Coagulation
Nanorods
Gold
Protein Kinases
Peptides
nanorods
peptides
Assays
gold
proteins
Proteins
phosphorylation
Phosphorylation
Substrates
Surface charge
inhibitors
bromides
absorptivity

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods. / Kitazaki, Hirotaro; Mori, Takeshi; Kang, Jeong Hun; Niidome, Takuro; Murata, Masaharu; Hashizume, Makoto; Katayama, Yoshiki.

In: Colloids and Surfaces B: Biointerfaces, Vol. 99, 01.11.2012, p. 7-11.

Research output: Contribution to journalArticle

Kitazaki, Hirotaro ; Mori, Takeshi ; Kang, Jeong Hun ; Niidome, Takuro ; Murata, Masaharu ; Hashizume, Makoto ; Katayama, Yoshiki. / A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods. In: Colloids and Surfaces B: Biointerfaces. 2012 ; Vol. 99. pp. 7-11.
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