A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods

Hirotaro Kitazaki, Takeshi Mori, Jeong Hun Kang, Takuro Niidome, Masaharu Murata, Makoto Hashizume, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We succeeded in applying gold nanorods (NRs) for the detection of protein kinase (PK) activity based on a substrate-induced coagulation mechanism. Because NRs have cationic surface charges due to surface-adsorbed cetyltrimethylammonium bromide, phosphorylated substrate peptides, which gain augmented anionic charges by phosphorylation, induce coagulation of the NRs, resulting in a red-to-blue color change of the corresponding NR dispersion. A large absorption coefficient of the NRs at the near-IR region enabled clear-cut detection of the peptide-induced coagulation. IC 50 values of several inhibitors of c-Src determined by the present assay are consistent with reported values.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume99
DOIs
Publication statusPublished - Nov 1 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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