TY - JOUR
T1 - A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods
AU - Kitazaki, Hirotaro
AU - Mori, Takeshi
AU - Kang, Jeong Hun
AU - Niidome, Takuro
AU - Murata, Masaharu
AU - Hashizume, Makoto
AU - Katayama, Yoshiki
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, and by a Health Labour Sciences Research Grant from Ministry of Health, Labour and Welfare.
PY - 2012/11/1
Y1 - 2012/11/1
N2 - We succeeded in applying gold nanorods (NRs) for the detection of protein kinase (PK) activity based on a substrate-induced coagulation mechanism. Because NRs have cationic surface charges due to surface-adsorbed cetyltrimethylammonium bromide, phosphorylated substrate peptides, which gain augmented anionic charges by phosphorylation, induce coagulation of the NRs, resulting in a red-to-blue color change of the corresponding NR dispersion. A large absorption coefficient of the NRs at the near-IR region enabled clear-cut detection of the peptide-induced coagulation. IC 50 values of several inhibitors of c-Src determined by the present assay are consistent with reported values.
AB - We succeeded in applying gold nanorods (NRs) for the detection of protein kinase (PK) activity based on a substrate-induced coagulation mechanism. Because NRs have cationic surface charges due to surface-adsorbed cetyltrimethylammonium bromide, phosphorylated substrate peptides, which gain augmented anionic charges by phosphorylation, induce coagulation of the NRs, resulting in a red-to-blue color change of the corresponding NR dispersion. A large absorption coefficient of the NRs at the near-IR region enabled clear-cut detection of the peptide-induced coagulation. IC 50 values of several inhibitors of c-Src determined by the present assay are consistent with reported values.
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U2 - 10.1016/j.colsurfb.2011.08.028
DO - 10.1016/j.colsurfb.2011.08.028
M3 - Article
C2 - 21944544
AN - SCOPUS:84863860927
SN - 0927-7765
VL - 99
SP - 7
EP - 11
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -