A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma

Yoshiaki Kinoshita, Tomoyuki Hida, Makoto Hamasaki, Shinji Matsumoto, Ayuko Sato, Tohru Tsujimura, Kunimitsu Kawahara, Kenzo Hiroshima, Yoshinao Oda, Kazuki Nabeshima

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

BACKGROUND: Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors previously described that IHC expression of the protein product of the methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 chromosomal region, was correlated with the deletion status of 9p21 FISH in MPM tissues. In the current study, the authors investigated whether a combination of MTAP and BAP1 IHC could distinguish MPM from reactive mesothelial cells (RMC) in cell blocks obtained from pleural effusions. METHODS: The authors examined IHC expression of MTAP and BAP1 in cell blocks obtained from pleural effusions of 45 cases of MPM and 21 cases of reactive mesothelial hyperplasia. Furthermore, IHC expression of MTAP was compared with the deletion status of 9p21 FISH. RESULTS: MTAP and BAP1 IHC differentiated MPM from RMC with 100% specificity for both and sensitivities of 42.2% and 60.0%, respectively. The combination of MTAP and BAP1 IHC yielded a sensitivity of 77.8%, which was higher than that of BAP1 IHC alone or 9p21 FISH alone (62.2%). Moreover, a high degree of concordance was observed between the results of MTAP IHC and 9p21 FISH in cell blocks. CONCLUSIONS: A combination of MTAP and BAP1 IHC in cell blocks from pleural effusions appears to be a reliable and useful method for differentiating MPM cells from RMC and can be used in the routine diagnosis of MPM. Cancer Cytopathol 2018;126:54–63.

Original languageEnglish
Pages (from-to)54-63
Number of pages10
JournalCancer Cytopathology
Volume126
Issue number1
DOIs
Publication statusPublished - Jan 2018

Fingerprint

BRCA1 Protein
Mesothelioma
Pleural Effusion
Cell Biology
Immunohistochemistry
Fluorescence In Situ Hybridization
Hyperplasia
5'-methylthioadenosine phosphorylase
Malignant Mesothelioma

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kinoshita, Y., Hida, T., Hamasaki, M., Matsumoto, S., Sato, A., Tsujimura, T., ... Nabeshima, K. (2018). A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma. Cancer Cytopathology, 126(1), 54-63. https://doi.org/10.1002/cncy.21928

A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma. / Kinoshita, Yoshiaki; Hida, Tomoyuki; Hamasaki, Makoto; Matsumoto, Shinji; Sato, Ayuko; Tsujimura, Tohru; Kawahara, Kunimitsu; Hiroshima, Kenzo; Oda, Yoshinao; Nabeshima, Kazuki.

In: Cancer Cytopathology, Vol. 126, No. 1, 01.2018, p. 54-63.

Research output: Contribution to journalArticle

Kinoshita, Y, Hida, T, Hamasaki, M, Matsumoto, S, Sato, A, Tsujimura, T, Kawahara, K, Hiroshima, K, Oda, Y & Nabeshima, K 2018, 'A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma', Cancer Cytopathology, vol. 126, no. 1, pp. 54-63. https://doi.org/10.1002/cncy.21928
Kinoshita, Yoshiaki ; Hida, Tomoyuki ; Hamasaki, Makoto ; Matsumoto, Shinji ; Sato, Ayuko ; Tsujimura, Tohru ; Kawahara, Kunimitsu ; Hiroshima, Kenzo ; Oda, Yoshinao ; Nabeshima, Kazuki. / A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma. In: Cancer Cytopathology. 2018 ; Vol. 126, No. 1. pp. 54-63.
@article{82255445c4364ae894fc47c5c57b4cdc,
title = "A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma",
abstract = "BACKGROUND: Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors previously described that IHC expression of the protein product of the methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 chromosomal region, was correlated with the deletion status of 9p21 FISH in MPM tissues. In the current study, the authors investigated whether a combination of MTAP and BAP1 IHC could distinguish MPM from reactive mesothelial cells (RMC) in cell blocks obtained from pleural effusions. METHODS: The authors examined IHC expression of MTAP and BAP1 in cell blocks obtained from pleural effusions of 45 cases of MPM and 21 cases of reactive mesothelial hyperplasia. Furthermore, IHC expression of MTAP was compared with the deletion status of 9p21 FISH. RESULTS: MTAP and BAP1 IHC differentiated MPM from RMC with 100{\%} specificity for both and sensitivities of 42.2{\%} and 60.0{\%}, respectively. The combination of MTAP and BAP1 IHC yielded a sensitivity of 77.8{\%}, which was higher than that of BAP1 IHC alone or 9p21 FISH alone (62.2{\%}). Moreover, a high degree of concordance was observed between the results of MTAP IHC and 9p21 FISH in cell blocks. CONCLUSIONS: A combination of MTAP and BAP1 IHC in cell blocks from pleural effusions appears to be a reliable and useful method for differentiating MPM cells from RMC and can be used in the routine diagnosis of MPM. Cancer Cytopathol 2018;126:54–63.",
author = "Yoshiaki Kinoshita and Tomoyuki Hida and Makoto Hamasaki and Shinji Matsumoto and Ayuko Sato and Tohru Tsujimura and Kunimitsu Kawahara and Kenzo Hiroshima and Yoshinao Oda and Kazuki Nabeshima",
year = "2018",
month = "1",
doi = "10.1002/cncy.21928",
language = "English",
volume = "126",
pages = "54--63",
journal = "Cancer cytopathology",
issn = "1934-662X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - A combination of MTAP and BAP1 immunohistochemistry in pleural effusion cytology for the diagnosis of mesothelioma

AU - Kinoshita, Yoshiaki

AU - Hida, Tomoyuki

AU - Hamasaki, Makoto

AU - Matsumoto, Shinji

AU - Sato, Ayuko

AU - Tsujimura, Tohru

AU - Kawahara, Kunimitsu

AU - Hiroshima, Kenzo

AU - Oda, Yoshinao

AU - Nabeshima, Kazuki

PY - 2018/1

Y1 - 2018/1

N2 - BACKGROUND: Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors previously described that IHC expression of the protein product of the methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 chromosomal region, was correlated with the deletion status of 9p21 FISH in MPM tissues. In the current study, the authors investigated whether a combination of MTAP and BAP1 IHC could distinguish MPM from reactive mesothelial cells (RMC) in cell blocks obtained from pleural effusions. METHODS: The authors examined IHC expression of MTAP and BAP1 in cell blocks obtained from pleural effusions of 45 cases of MPM and 21 cases of reactive mesothelial hyperplasia. Furthermore, IHC expression of MTAP was compared with the deletion status of 9p21 FISH. RESULTS: MTAP and BAP1 IHC differentiated MPM from RMC with 100% specificity for both and sensitivities of 42.2% and 60.0%, respectively. The combination of MTAP and BAP1 IHC yielded a sensitivity of 77.8%, which was higher than that of BAP1 IHC alone or 9p21 FISH alone (62.2%). Moreover, a high degree of concordance was observed between the results of MTAP IHC and 9p21 FISH in cell blocks. CONCLUSIONS: A combination of MTAP and BAP1 IHC in cell blocks from pleural effusions appears to be a reliable and useful method for differentiating MPM cells from RMC and can be used in the routine diagnosis of MPM. Cancer Cytopathol 2018;126:54–63.

AB - BACKGROUND: Homozygous deletion of 9p21 detected by fluorescence in situ hybridization (FISH) and loss of BRCA1-associated protein 1 (BAP1) expression detected by immunohistochemistry (IHC) are useful for the differentiation between malignant pleural mesothelioma (MPM) and reactive mesothelial hyperplasia. The authors previously described that IHC expression of the protein product of the methylthioadenosine phosphorylase (MTAP) gene, which is localized in the 9p21 chromosomal region, was correlated with the deletion status of 9p21 FISH in MPM tissues. In the current study, the authors investigated whether a combination of MTAP and BAP1 IHC could distinguish MPM from reactive mesothelial cells (RMC) in cell blocks obtained from pleural effusions. METHODS: The authors examined IHC expression of MTAP and BAP1 in cell blocks obtained from pleural effusions of 45 cases of MPM and 21 cases of reactive mesothelial hyperplasia. Furthermore, IHC expression of MTAP was compared with the deletion status of 9p21 FISH. RESULTS: MTAP and BAP1 IHC differentiated MPM from RMC with 100% specificity for both and sensitivities of 42.2% and 60.0%, respectively. The combination of MTAP and BAP1 IHC yielded a sensitivity of 77.8%, which was higher than that of BAP1 IHC alone or 9p21 FISH alone (62.2%). Moreover, a high degree of concordance was observed between the results of MTAP IHC and 9p21 FISH in cell blocks. CONCLUSIONS: A combination of MTAP and BAP1 IHC in cell blocks from pleural effusions appears to be a reliable and useful method for differentiating MPM cells from RMC and can be used in the routine diagnosis of MPM. Cancer Cytopathol 2018;126:54–63.

UR - http://www.scopus.com/inward/record.url?scp=85031721158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85031721158&partnerID=8YFLogxK

U2 - 10.1002/cncy.21928

DO - 10.1002/cncy.21928

M3 - Article

C2 - 29053210

AN - SCOPUS:85031721158

VL - 126

SP - 54

EP - 63

JO - Cancer cytopathology

JF - Cancer cytopathology

SN - 1934-662X

IS - 1

ER -