A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures

Shinji Matsumoto, Shinsuke Fujii, Akira Sato, Souji Ibuka, Yoshinori Kagawa, Masaru Ishii, Akira Kikuchi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.

Original languageEnglish
Pages (from-to)702-718
Number of pages17
JournalEMBO Journal
Volume33
Issue number7
DOIs
Publication statusPublished - Apr 1 2014
Externally publishedYes

Fingerprint

Wnt Proteins
Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Organoids
Kidney
Fibroblast Growth Factors
Cell growth
Molecular Structure
Cytoskeleton
GTP-Binding Proteins
Elongation
Assays
Embryonic Structures
Epithelial Cells
Chemical activation
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. / Matsumoto, Shinji; Fujii, Shinsuke; Sato, Akira; Ibuka, Souji; Kagawa, Yoshinori; Ishii, Masaru; Kikuchi, Akira.

In: EMBO Journal, Vol. 33, No. 7, 01.04.2014, p. 702-718.

Research output: Contribution to journalArticle

Matsumoto, Shinji ; Fujii, Shinsuke ; Sato, Akira ; Ibuka, Souji ; Kagawa, Yoshinori ; Ishii, Masaru ; Kikuchi, Akira. / A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. In: EMBO Journal. 2014 ; Vol. 33, No. 7. pp. 702-718.
@article{fd81c1f94894418281af714ba267602e,
title = "A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures",
abstract = "Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.",
author = "Shinji Matsumoto and Shinsuke Fujii and Akira Sato and Souji Ibuka and Yoshinori Kagawa and Masaru Ishii and Akira Kikuchi",
year = "2014",
month = "4",
day = "1",
doi = "10.1002/embj.201386942",
language = "English",
volume = "33",
pages = "702--718",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures

AU - Matsumoto, Shinji

AU - Fujii, Shinsuke

AU - Sato, Akira

AU - Ibuka, Souji

AU - Kagawa, Yoshinori

AU - Ishii, Masaru

AU - Kikuchi, Akira

PY - 2014/4/1

Y1 - 2014/4/1

N2 - Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.

AB - Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.

UR - http://www.scopus.com/inward/record.url?scp=84898626731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898626731&partnerID=8YFLogxK

U2 - 10.1002/embj.201386942

DO - 10.1002/embj.201386942

M3 - Article

C2 - 24562386

AN - SCOPUS:84898626731

VL - 33

SP - 702

EP - 718

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 7

ER -