A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors.

Daisuke Kohda, C. Kodama, R. Kase, H. Nomoto, K. Hayashi, F. Inagaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The three-dimensional structure of the mouse epidermal growth factor (EGF) in solution was studied by comparison of the 1H NMR spectra of alpha EGF (1-53) and beta EGF (2-53, des-asparaginyl 1 form). Using pH dependence of chemical shifts and a two-dimensional difference spectrum, the effect of the N-terminal deletion was investigated based on the complete assignment of the proton resonances. The affected residues were all found to be located exactly in the triple-stranded, beta-sheet core in the N-terminal domain of the EGF molecule.

Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalBiochemistry international
Volume16
Issue number4
Publication statusPublished - Jan 1 1988

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Epidermal Growth Factor
Nuclear magnetic resonance
Chemical shift
Protons
Molecules
EGF Family of Proteins
Proton Magnetic Resonance Spectroscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Kohda, D., Kodama, C., Kase, R., Nomoto, H., Hayashi, K., & Inagaki, F. (1988). A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors. Biochemistry international, 16(4), 647-654.

A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors. / Kohda, Daisuke; Kodama, C.; Kase, R.; Nomoto, H.; Hayashi, K.; Inagaki, F.

In: Biochemistry international, Vol. 16, No. 4, 01.01.1988, p. 647-654.

Research output: Contribution to journalArticle

Kohda, D, Kodama, C, Kase, R, Nomoto, H, Hayashi, K & Inagaki, F 1988, 'A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors.', Biochemistry international, vol. 16, no. 4, pp. 647-654.
Kohda, Daisuke ; Kodama, C. ; Kase, R. ; Nomoto, H. ; Hayashi, K. ; Inagaki, F. / A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors. In: Biochemistry international. 1988 ; Vol. 16, No. 4. pp. 647-654.
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