A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors.

D. Kohda, C. Kodama, R. Kase, H. Nomoto, K. Hayashi, F. Inagaki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The three-dimensional structure of the mouse epidermal growth factor (EGF) in solution was studied by comparison of the 1H NMR spectra of alpha EGF (1-53) and beta EGF (2-53, des-asparaginyl 1 form). Using pH dependence of chemical shifts and a two-dimensional difference spectrum, the effect of the N-terminal deletion was investigated based on the complete assignment of the proton resonances. The affected residues were all found to be located exactly in the triple-stranded, beta-sheet core in the N-terminal domain of the EGF molecule.

Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalBiochemistry international
Volume16
Issue number4
Publication statusPublished - Apr 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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