A comparative study of the enzymologieal features of α-amylase in the Drosophila melanogaster species subgroup

Hiroki Shibata, Tsuneyuki Yamazaki

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12 Citations (Scopus)

Abstract

A comparative study was done on the enzymologieal features of α-amylase among six sibling species belonging to the Drosophila melanogaster species subgroup. The pH optima were 7.6 for D. melanogaster, D. mauritiana and D. simulans and 7.4 for D. erecta, D. teissieri and D. yakuba. The temperature optima were commonly 37 C in all species studied. However, there are two types of temperature dependence; a strict one as observed in D. melanogaster, D. mauritiana and D. simulans, and a flat one as observed in D. erecta, D. teissieri and D. yakuba. These separations are consistent with the general phylogeny within this species subgroup. The substrate dependence was almost the same in all the species studied. The imax and Km were also estimated for each species. We found clear differences in the enzymologieal features between the species. Thus these differences might reflect an adaptation of the amylase enzyme system to speciation in this species subgroup.

Original languageEnglish
Pages (from-to)251-258
Number of pages8
JournalJapanese Journal of Genetics
Volume69
Issue number3
DOIs
Publication statusPublished - Jan 1 1994

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Amylases
Drosophila melanogaster
Temperature
Phylogeny
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics

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A comparative study of the enzymologieal features of α-amylase in the Drosophila melanogaster species subgroup. / Shibata, Hiroki; Yamazaki, Tsuneyuki.

In: Japanese Journal of Genetics, Vol. 69, No. 3, 01.01.1994, p. 251-258.

Research output: Contribution to journalArticle

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