A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q

Yoichi Yamada, Hidemi Watanabe, Fumihito Miura, Hidenobu Soejima, Michiko Uchiyama, Tsuyoshi Iwasaka, Tsunehiro Mukai, Yoshiyuki Sakaki, Takashi Ito

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Approximately half of all human genes have CpG islands (CGIs) around their promoter regions. Although CGIs usually escape methylation, those on Chromosome X in females and those in the vicinity of imprinted genes are exceptions: They have both methylated and unmethylated alleles to display a "composite" pattern in methylation analysis. In addition, aberrant methylation of CGIs is known to often occur in cancer cells. Here we developed a simple Hpall-McrBC PCR method for discrimination of full, null, incomplete, and composite methylation patterns, and applied it to all computationally identified CGIs on human Chromosome 21q. This comprehensive analysis revealed that, although most CGIs (103 out of 149) escape methylation, a sizable fraction (31 out of 149) are fully methylated even in normal peripheral blood cells. Furthermore, we identified seven CGIs showing the composite methylation, and demonstrated that three of them are indeed methylated monoallelically. Further analyses using informative pedigrees revealed that two of the three are subject to maternal allele-specific methylation. Intriguingly, the other CGI is methylated in an allele-specific but parental-origin-independent manner. Thus, the cell seems to have a broader repertoire of methylating CGIs than previously thought, and our approach may contribute to uncover novel modes of allelic methylation.

Original languageEnglish
Pages (from-to)247-266
Number of pages20
JournalGenome Research
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 1 2004
Externally publishedYes

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CpG Islands
Human Chromosomes
Methylation
Alleles
X Chromosome
Pedigree
Genetic Promoter Regions
Genes
Blood Cells
Mothers
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q. / Yamada, Yoichi; Watanabe, Hidemi; Miura, Fumihito; Soejima, Hidenobu; Uchiyama, Michiko; Iwasaka, Tsuyoshi; Mukai, Tsunehiro; Sakaki, Yoshiyuki; Ito, Takashi.

In: Genome Research, Vol. 14, No. 2, 01.02.2004, p. 247-266.

Research output: Contribution to journalArticle

Yamada, Y, Watanabe, H, Miura, F, Soejima, H, Uchiyama, M, Iwasaka, T, Mukai, T, Sakaki, Y & Ito, T 2004, 'A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q', Genome Research, vol. 14, no. 2, pp. 247-266. https://doi.org/10.1101/gr.1351604
Yamada, Yoichi ; Watanabe, Hidemi ; Miura, Fumihito ; Soejima, Hidenobu ; Uchiyama, Michiko ; Iwasaka, Tsuyoshi ; Mukai, Tsunehiro ; Sakaki, Yoshiyuki ; Ito, Takashi. / A comprehensive analysis of allelic methylation status of CpG islands on human chromosome 21q. In: Genome Research. 2004 ; Vol. 14, No. 2. pp. 247-266.
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