Abstract
To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about 9,200 expression sequence tag fragments and corresponded to about 7,000 genes. We found that log phase cells predominantly expressed DNA/ chromosome duplication gene homologs. In addition, many genes for basic transcription and translation machineries, as well as proteasomal genes, were up-regulated at the log phase. About half of the kinesin homolog genes, but not myosin homolog genes, were predominantly expressed at the dividing phase as well. In contrast, stationary phase cells expressed genes for many receptor kinases, signal transduction machineries and transcription factors. Several hundreds of genes showed differential expression after incubation of stationary phase cells with medium containing either salicylic acid or abscisic acid. These findings suggested that BY-2 cells at the stationary phase express genes for perceiving extracellular signals.
| Original language | English |
|---|---|
| Pages (from-to) | 1280-1289 |
| Number of pages | 10 |
| Journal | Plant and Cell Physiology |
| Volume | 45 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Jan 1 2004 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Plant Science
- Cell Biology
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A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells. / Matsuoka, Ken; Demura, Taku; Galis, Ivan; Horiguchi, Tatsuya; Sasaki, Mami; Tashiro, Gen; Fukuda, Hiroo.
In: Plant and Cell Physiology, Vol. 45, No. 9, 01.01.2004, p. 1280-1289.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells
AU - Matsuoka, Ken
AU - Demura, Taku
AU - Galis, Ivan
AU - Horiguchi, Tatsuya
AU - Sasaki, Mami
AU - Tashiro, Gen
AU - Fukuda, Hiroo
PY - 2004/1/1
Y1 - 2004/1/1
N2 - To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about 9,200 expression sequence tag fragments and corresponded to about 7,000 genes. We found that log phase cells predominantly expressed DNA/ chromosome duplication gene homologs. In addition, many genes for basic transcription and translation machineries, as well as proteasomal genes, were up-regulated at the log phase. About half of the kinesin homolog genes, but not myosin homolog genes, were predominantly expressed at the dividing phase as well. In contrast, stationary phase cells expressed genes for many receptor kinases, signal transduction machineries and transcription factors. Several hundreds of genes showed differential expression after incubation of stationary phase cells with medium containing either salicylic acid or abscisic acid. These findings suggested that BY-2 cells at the stationary phase express genes for perceiving extracellular signals.
AB - To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about 9,200 expression sequence tag fragments and corresponded to about 7,000 genes. We found that log phase cells predominantly expressed DNA/ chromosome duplication gene homologs. In addition, many genes for basic transcription and translation machineries, as well as proteasomal genes, were up-regulated at the log phase. About half of the kinesin homolog genes, but not myosin homolog genes, were predominantly expressed at the dividing phase as well. In contrast, stationary phase cells expressed genes for many receptor kinases, signal transduction machineries and transcription factors. Several hundreds of genes showed differential expression after incubation of stationary phase cells with medium containing either salicylic acid or abscisic acid. These findings suggested that BY-2 cells at the stationary phase express genes for perceiving extracellular signals.
UR - http://www.scopus.com/inward/record.url?scp=7944237619&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7944237619&partnerID=8YFLogxK
U2 - 10.1093/pcp/pch155
DO - 10.1093/pcp/pch155
M3 - Article
C2 - 15509851
AN - SCOPUS:7944237619
VL - 45
SP - 1280
EP - 1289
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 9
ER -