A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells

Ken Matsuoka, Taku Demura, Ivan Galis, Tatsuya Horiguchi, Mami Sasaki, Gen Tashiro, Hiroo Fukuda

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

To understand how plant cell changes gene expression during cell division and after termination of cell division, we analyzed the change of gene expression during the growth of tobacco BY-2 cell lines using a cDNA microarray, which contained about 9,200 expression sequence tag fragments and corresponded to about 7,000 genes. We found that log phase cells predominantly expressed DNA/ chromosome duplication gene homologs. In addition, many genes for basic transcription and translation machineries, as well as proteasomal genes, were up-regulated at the log phase. About half of the kinesin homolog genes, but not myosin homolog genes, were predominantly expressed at the dividing phase as well. In contrast, stationary phase cells expressed genes for many receptor kinases, signal transduction machineries and transcription factors. Several hundreds of genes showed differential expression after incubation of stationary phase cells with medium containing either salicylic acid or abscisic acid. These findings suggested that BY-2 cells at the stationary phase express genes for perceiving extracellular signals.

Original languageEnglish
Pages (from-to)1280-1289
Number of pages10
JournalPlant and Cell Physiology
Volume45
Issue number9
DOIs
Publication statusPublished - Jan 1 2004

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Tobacco
tobacco
Gene Expression
gene expression
Growth
Genes
genes
cells
cell division
Cell Division
Chromosome Duplication
kinesin
Kinesin
Abscisic Acid
gene duplication
Salicylic Acid
myosin
salicylic acid
Plant Cells
Myosins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells. / Matsuoka, Ken; Demura, Taku; Galis, Ivan; Horiguchi, Tatsuya; Sasaki, Mami; Tashiro, Gen; Fukuda, Hiroo.

In: Plant and Cell Physiology, Vol. 45, No. 9, 01.01.2004, p. 1280-1289.

Research output: Contribution to journalArticle

Matsuoka, Ken ; Demura, Taku ; Galis, Ivan ; Horiguchi, Tatsuya ; Sasaki, Mami ; Tashiro, Gen ; Fukuda, Hiroo. / A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 9. pp. 1280-1289.
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