TY - JOUR
T1 - A comprehensive study on rational biocatalysts and individual components of photobiocatalytic H2 production systems
AU - Kosem, Nuttavut
AU - Watanabe, Motonori
AU - Song, Juntae
AU - Takagaki, Atsushi
AU - Ishihara, Tatsumi
N1 - Funding Information:
This study was financially supported by a Grant-in-Aid for Specially Promoted Research (No. 21K18213 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan through the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/2/5
Y1 - 2023/2/5
N2 - Photobiocatalytic system has been developing as a promising approach for H2 production. Herein, the rational characteristics of biocatalysts and the role of individual components affecting the efficiency of the system were investigated. Photocatalytic studies showed that tris (2-amino-2-hydroxymethyl-1,3-propanediol) was an ideal electron donor for viologen reduction by TiO2. Biocatalytic reaction revealed that cell permeability, the redox potential of electron mediators and the cell envelope were crucial to the activity of whole-cell biocatalysts. In photobiocatalytic system, recombinant Escherichia coli with a turnover frequency of 39.43 ± 3.77 s−1 based on [FeFe]-hydrogenase activity was a more rational biocatalyst than Anabaena variabilis. A comprehensive study found that the presence of TiO2, light and biocatalysts strongly enhanced H2 production, whereas Tris and MV2+ had less influence. A maximum rate was found at 16.73 ± 1.03 μmol/min with a solar-to-H2 conversion of 1.58 ± 0.10 %. Understanding the role of each component will guide the development of high-efficient photobiocatalysis.
AB - Photobiocatalytic system has been developing as a promising approach for H2 production. Herein, the rational characteristics of biocatalysts and the role of individual components affecting the efficiency of the system were investigated. Photocatalytic studies showed that tris (2-amino-2-hydroxymethyl-1,3-propanediol) was an ideal electron donor for viologen reduction by TiO2. Biocatalytic reaction revealed that cell permeability, the redox potential of electron mediators and the cell envelope were crucial to the activity of whole-cell biocatalysts. In photobiocatalytic system, recombinant Escherichia coli with a turnover frequency of 39.43 ± 3.77 s−1 based on [FeFe]-hydrogenase activity was a more rational biocatalyst than Anabaena variabilis. A comprehensive study found that the presence of TiO2, light and biocatalysts strongly enhanced H2 production, whereas Tris and MV2+ had less influence. A maximum rate was found at 16.73 ± 1.03 μmol/min with a solar-to-H2 conversion of 1.58 ± 0.10 %. Understanding the role of each component will guide the development of high-efficient photobiocatalysis.
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U2 - 10.1016/j.apcata.2022.119019
DO - 10.1016/j.apcata.2022.119019
M3 - Article
AN - SCOPUS:85147108820
SN - 0926-860X
VL - 651
JO - Applied Catalysis A: General
JF - Applied Catalysis A: General
M1 - 119019
ER -