A comprehensive two-hybrid analysis to explore the yeast protein interactome

Takashi Ito, Tomoko Chiba, Ritsuko Ozawa, Mikio Yoshida, Masahira Hattori, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

2748 Citations (Scopus)

Abstract

Protein-protein interactions play crucial roles in the execution of various biological functions. Accordingly, their comprehensive description would contribute considerably to the functional interpretation of fully sequenced genomes, which are flooded with novel genes of unpredictable functions. We previously developed a system to examine two-hybrid interactions in all possible combinations between the ≈6,000 proteins of the budding yeast Saccharomyces cerevisiae. Here we have completed the comprehensive analysis using this system to identify 4,549 two-hybrid interactions among 3,278 proteins. Unexpectedly, these data do not largely overlap with those obtained by the other project [Uetz, P., et al. (2000) Nature (London) 403, 623-627] and hence have substantially expanded our knowledge on the protein interaction space or interactome of the yeast. Cumulative connection of these binary interactions generates a single huge network linking the vast majority of the proteins. Bioinformatics-aided selection of biologically relevant interactions highlights various intriguing sub-networks. They include, for instance, the one that had successfully foreseen the involvement of a novel protein in spindle pole body function as well as the one that may uncover a hitherto unidentified multiprotein complex potentially participating in the process of vesicular transport. Our data would thus significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.

Original languageEnglish
Pages (from-to)4569-4574
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number8
DOIs
Publication statusPublished - Apr 10 2001

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Fungal Proteins
Proteins
Spindle Pole Bodies
Genome
Protein Interaction Maps
Multiprotein Complexes
Saccharomycetales
Computational Biology
Saccharomyces cerevisiae
Yeasts
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

A comprehensive two-hybrid analysis to explore the yeast protein interactome. / Ito, Takashi; Chiba, Tomoko; Ozawa, Ritsuko; Yoshida, Mikio; Hattori, Masahira; Sakaki, Yoshiyuki.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 8, 10.04.2001, p. 4569-4574.

Research output: Contribution to journalArticle

Ito, Takashi ; Chiba, Tomoko ; Ozawa, Ritsuko ; Yoshida, Mikio ; Hattori, Masahira ; Sakaki, Yoshiyuki. / A comprehensive two-hybrid analysis to explore the yeast protein interactome. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 8. pp. 4569-4574.
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