A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255

Chizuru Kinoshita-Iramina, Maki Kit Ahara, Katsumi Doi, Seiya Ogata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.

Original languageEnglish
Pages (from-to)1469-1473
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number9
DOIs
Publication statusPublished - Jan 1 1997

Fingerprint

Streptomyces
Plasmids
Thiostrepton
Mycelium
DNA
Spores
Productivity
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255. / Kinoshita-Iramina, Chizuru; Ahara, Maki Kit; Doi, Katsumi; Ogata, Seiya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 9, 01.01.1997, p. 1469-1473.

Research output: Contribution to journalArticle

Kinoshita-Iramina, Chizuru ; Ahara, Maki Kit ; Doi, Katsumi ; Ogata, Seiya. / A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255. In: Bioscience, Biotechnology and Biochemistry. 1997 ; Vol. 61, No. 9. pp. 1469-1473.
@article{e7e12d1f2b9a4ceca04a08b3d5553b74,
title = "A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255",
abstract = "Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.",
author = "Chizuru Kinoshita-Iramina and Ahara, {Maki Kit} and Katsumi Doi and Seiya Ogata",
year = "1997",
month = "1",
day = "1",
doi = "10.1271/bbb.61.1469",
language = "English",
volume = "61",
pages = "1469--1473",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "9",

}

TY - JOUR

T1 - A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255

AU - Kinoshita-Iramina, Chizuru

AU - Ahara, Maki Kit

AU - Doi, Katsumi

AU - Ogata, Seiya

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.

AB - Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.

UR - http://www.scopus.com/inward/record.url?scp=0031217870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031217870&partnerID=8YFLogxK

U2 - 10.1271/bbb.61.1469

DO - 10.1271/bbb.61.1469

M3 - Article

VL - 61

SP - 1469

EP - 1473

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 9

ER -