A Conjugative Linear Plasmid in Streptomyces laurentii ATCC31255

Chizuru Kinoshita-Iramina, Maki Kit Ahara, Katsumi Doi, Seiya Ogata

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Abstract

Plasmid pSLL of Streptomyces laurentii ATCC31255 (wild-type strain P0) is a 93-kilobase linear DNA plasmid that carries a protein bound to each 5′ end of the DNA. It was self-transmitted to the pSLL-cured strain by conjugation in solid culture. The pSLL-cured strain carried a circular plasmid, pSLS, and showed a marked decrease in spore formation and thiostrepton productivity, owing to the pSLS. However, by retransmission of pSLL, these things reverted to levels seen in strain PO. Thus, plasmid pSLL suppressed the injurious effects of pSLS on the host mycelia.

Original languageEnglish
Pages (from-to)1469-1473
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number9
DOIs
Publication statusPublished - Jan 1997

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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