A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm

Lihong Tian, Yanping Xing, Masako Fukuda, Rong Li, Toshihiro Kumamaru, Dandan Qian, Xiangbai Dong, Le Qing Qu

Research output: Contribution to journalArticle

Abstract

Rice glutelins are initially synthesized as 57-kDa precursors at the endoplasmic reticulum (ER) and are ultimately transported into protein storage vacuoles. However, the sequence motifs that affect proglutelin folding, assembly, and their export from the ER remain poorly defined. In this study, we characterized a mutant with nine amino acids deleted in the GluA2 protein, which resulted in specific accumulation of the GluA precursor. The deleted amino acids constitute a well-conserved sequence (LVYIIQGRG) in glutelins and all residues in this motif are necessary for ER export of GluA2. Immunoelectron microscopy and stable transgenic analyses indicated that proglutelins with deletion of this motif misassembled and aggregated through non-native intermolecular disulfide bonds, and were deposited in ER-derived protein bodies (PB-Is), resulting in conversion of PB-Is into a new type of PB. These results indicate that the conserved motif is essential for proper assembly of proglutelin. The correct assembly of proglutelins is critical for their segregation from prolamins in the ER lumen, which is essential for enabling the export of proglutelin from the ER and for the proper formation of PB-Is. We also found that the interchain disulfide bond between acidic and basic subunits is not necessary for their assembly, but it is required for proglutelin folding.

Original languageEnglish
Pages (from-to)5029-5043
Number of pages15
JournalJournal of Experimental Botany
Volume69
Issue number21
DOIs
Publication statusPublished - Oct 12 2018

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Endosperm
Endoplasmic Reticulum
endoplasmic reticulum
endosperm
rice
glutelins
protein bodies
Glutens
disulfide bonds
Disulfides
Proteins
Prolamins
Amino Acids
amino acids
prolamins
Immunoelectron Microscopy
conserved sequences
Conserved Sequence
storage proteins
Vacuoles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm. / Tian, Lihong; Xing, Yanping; Fukuda, Masako; Li, Rong; Kumamaru, Toshihiro; Qian, Dandan; Dong, Xiangbai; Qu, Le Qing.

In: Journal of Experimental Botany, Vol. 69, No. 21, 12.10.2018, p. 5029-5043.

Research output: Contribution to journalArticle

Tian, Lihong ; Xing, Yanping ; Fukuda, Masako ; Li, Rong ; Kumamaru, Toshihiro ; Qian, Dandan ; Dong, Xiangbai ; Qu, Le Qing. / A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm. In: Journal of Experimental Botany. 2018 ; Vol. 69, No. 21. pp. 5029-5043.
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