A critical period requiring Rho proteins for cell cycle progression uncovered by reversible protein transduction in endothelial cells

Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Hideo Kanaide

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The time-specific requirement of Rho proteins for the S phase progression of vascular endothelial cells was determined by reversibly introducing inhibitor proteins with a cell-penetrating peptide. We found evidence of the reversibility of protein transduction. The removal of extracellular protein caused the transduced protein to decay in a manner sensitive to low temperatures. The time required for a 50% decay correlated with the protein size. The time-specific transduction of the inhibitor proteins uncovered a critical period requiring Rho proteins in the G1-S transition phase. Reversible protein transduction may thus be a powerful tool to investigate the time-specific role of signaling proteins.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalFEBS Letters
Volume570
Issue number1-3
DOIs
Publication statusPublished - Jul 16 2004

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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