A Critical Role of the 3′ Terminus of Nascent DNA Chains in Recognition of Stalled Replication Forks

Toshimi Mizukoshi, Taku Tanaka, Ken Ichi Arai, Daisuke Kohda, Hisao Masai

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Arrest of replication forks by various internal and external threats evokes a myriad of cellular reactions, collectively known as DNA replication checkpoint responses. In bacteria, PriA is essential for restoration of stalled replication forks and recombinational repair of double-stranded DNA breaks and is a candidate sensor protein that may recognize arrested forks. Here, we report that PriA protein specifically recognizes 3′ termini of arrested nascent DNA chains at model stalled replication forks in vitro. Mutations in the putative "3′ terminus binding pocket" present in the N-terminal segment of PriA result in reduced binding to stalled replication fork structures and loss of its biological functions. The results suggest a mechanism by which stalled replication forks are recognized by a sensor protein for checkpoint responses.

    Original languageEnglish
    Pages (from-to)42234-42239
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume278
    Issue number43
    DOIs
    Publication statusPublished - Oct 24 2003

    Fingerprint

    DNA
    Proteins
    Double-Stranded DNA Breaks
    Sensors
    DNA Replication
    Restoration
    Bacteria
    Repair
    Mutation
    In Vitro Techniques

    All Science Journal Classification (ASJC) codes

    • Biochemistry

    Cite this

    A Critical Role of the 3′ Terminus of Nascent DNA Chains in Recognition of Stalled Replication Forks. / Mizukoshi, Toshimi; Tanaka, Taku; Arai, Ken Ichi; Kohda, Daisuke; Masai, Hisao.

    In: Journal of Biological Chemistry, Vol. 278, No. 43, 24.10.2003, p. 42234-42239.

    Research output: Contribution to journalArticle

    Mizukoshi, Toshimi ; Tanaka, Taku ; Arai, Ken Ichi ; Kohda, Daisuke ; Masai, Hisao. / A Critical Role of the 3′ Terminus of Nascent DNA Chains in Recognition of Stalled Replication Forks. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 43. pp. 42234-42239.
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