A Critical Role of the 3′ Terminus of Nascent DNA Chains in Recognition of Stalled Replication Forks

Toshimi Mizukoshi, Taku Tanaka, Ken Ichi Arai, Daisuke Kohda, Hisao Masai

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Arrest of replication forks by various internal and external threats evokes a myriad of cellular reactions, collectively known as DNA replication checkpoint responses. In bacteria, PriA is essential for restoration of stalled replication forks and recombinational repair of double-stranded DNA breaks and is a candidate sensor protein that may recognize arrested forks. Here, we report that PriA protein specifically recognizes 3′ termini of arrested nascent DNA chains at model stalled replication forks in vitro. Mutations in the putative "3′ terminus binding pocket" present in the N-terminal segment of PriA result in reduced binding to stalled replication fork structures and loss of its biological functions. The results suggest a mechanism by which stalled replication forks are recognized by a sensor protein for checkpoint responses.

Original languageEnglish
Pages (from-to)42234-42239
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number43
DOIs
Publication statusPublished - Oct 24 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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