A cryptic fimbrial gene in Serratia marcescens

T. Moriya, Shun-Ichiro Kawabata, Y. Mizunoe, K. Amako

Research output: Contribution to journalArticle

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Abstract

The gene coding for the mannose-sensitive hemagglutinating fimbriae in Serratia marcescens US5 was cloned into Escherichia coli K4 with a cosmid vector system. One of the transformants, US5-1, expressed two morphologically distinct fimbriae, one that was 5-nm wide and one that was 3-nm wide. The latter fimbria was morphologically and serologically indistinguishable from that of strain US5. Genetic analysis of transformant US5-1 showed that the gene responsible for the 5-nm-wide fimbriae was located more than 10 kilobases away from the gene responsible for the 3-nm-wide fimbriae. The molecular size of the subunits of these two fimbriae, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were 19 kilodaltons for the 3-nm-wide fimbriae and 20 kilodaltons for the 5-nm-wide fimbriae. Serologically, the 5-nm-wide fimbriae did not cross-react with monoclonal or polyclonal antibodies raised against the mannose-sensitive hemagglutinating fimbriae of strain US5. Strain EL101, which expressed only the 5-nm-wide fimbriae, did not agglutinate chicken or human erythrocytes. These experimental results suggest that the gene for the 5-nm-wide fimbriae is cryptic in strain US5 and is expressed in E. coli K4 only after it is moved by transformation.

Original languageEnglish
Pages (from-to)6629-6636
Number of pages8
JournalJournal of bacteriology
Volume171
Issue number12
DOIs
Publication statusPublished - Jan 1 1989

Fingerprint

Serratia marcescens
Mannose
Genes
Escherichia coli
Cosmids
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Chickens
Erythrocytes
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

A cryptic fimbrial gene in Serratia marcescens. / Moriya, T.; Kawabata, Shun-Ichiro; Mizunoe, Y.; Amako, K.

In: Journal of bacteriology, Vol. 171, No. 12, 01.01.1989, p. 6629-6636.

Research output: Contribution to journalArticle

Moriya, T. ; Kawabata, Shun-Ichiro ; Mizunoe, Y. ; Amako, K. / A cryptic fimbrial gene in Serratia marcescens. In: Journal of bacteriology. 1989 ; Vol. 171, No. 12. pp. 6629-6636.
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