A cyanobacterial non-coding RNA, Yfr1, is required for growth under multiple stress conditions

Takahiro Nakamura, Kumiko Naito, Naoto Yokota, Chieko Sugita, Mamoru Sugita

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Small, regulatory, non-coding RNA (ncRNA) is involved in various cell functions in both prokaryotes and eukaryotes. However, information on ncRNA in cyanobacteria is still scarce. We studied ncRNA genes by computational screening to compare the intergenic regions of the Synechococcus elongatus PCC 6301 genome with the genomes of three freshwater cyanobacteria. We identified an ncRNA gene in S. elongatus, which has been previously described as yfr1 in marine cyanobacteria. The S. elongatus yfr1 gene is 65 nucleotides long and is positioned between guaB and trxA. We found a high conservation of the yfr1 gene in most cyanobacterial lineages. A yfr1-deficient mutant showed reduced growth under various stress conditions, e.g. oxidative stress and high salt stress conditions, and showed unusual accumulation of sbtA mRNA. A gel shift assay demonstrated interaction of the Yfr1 RNA with sbtA mRNA in vitro. This suggests that the sbtA transcript is a target RNA for the Yfr1 RNA.

Original languageEnglish
Pages (from-to)1309-1318
Number of pages10
JournalPlant and Cell Physiology
Volume48
Issue number9
DOIs
Publication statusPublished - Sep 1 2007

Fingerprint

Untranslated RNA
Synechococcus elongatus
Cyanobacteria
RNA
Growth
Genes
genes
Genome
Synechococcus
Small Untranslated RNA
Messenger RNA
Intergenic DNA
genome
prokaryotic cells
Eukaryota
Fresh Water
intergenic DNA
salt stress
eukaryotic cells
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

A cyanobacterial non-coding RNA, Yfr1, is required for growth under multiple stress conditions. / Nakamura, Takahiro; Naito, Kumiko; Yokota, Naoto; Sugita, Chieko; Sugita, Mamoru.

In: Plant and Cell Physiology, Vol. 48, No. 9, 01.09.2007, p. 1309-1318.

Research output: Contribution to journalArticle

Nakamura, Takahiro ; Naito, Kumiko ; Yokota, Naoto ; Sugita, Chieko ; Sugita, Mamoru. / A cyanobacterial non-coding RNA, Yfr1, is required for growth under multiple stress conditions. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 9. pp. 1309-1318.
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