A cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells

Sok Keng Tong, Karen Mouriec, Ming Wei Kuo, Elisabeth Pellegrini, Marie Madeleine Gueguen, François Brion, Olivier Kah, Bon Chu Chung

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Aromatase is an enzyme that catalyzes the synthesis of estrogen in gonads and brain. Teleost fish express aromatase (AroB) strongly in the brain facilitating its detailed examination. To understand the function of AroB in the brain, we generated transgenic zebrafish that expresses green fluorescent protein (GFP) driven by the brain aromatase cyp19a1b promoter. GFP was found in the radial glial cells of transgenic larvae and adult fish that overlap with AroB immunoreactivity in the correct temporal and spatial pattern. GFP was also coexpressed with radial cell marker BLBP, but was not in neurons. In addition, GFP expression in the radial glial cells was stimulated by estrogen, same as endogenous AroB expression. Thus, this transgenic line faithfully mimics the regulation of AroB expression in radial glial cells. It provides a powerful tool to further characterize progenitor radial cells in adult and developing fish and to evaluate estrogenic activities of xenoestrogens and phytoestrogens.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalGenesis
Volume47
Issue number2
DOIs
Publication statusPublished - Jul 1 2009

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Ependymoglial Cells
Aromatase
Zebrafish
Green Fluorescent Proteins
Fishes
Brain
Estrogens
Phytoestrogens
Gonads
Larva
Stem Cells
Neurons
IgA receptor
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Tong, S. K., Mouriec, K., Kuo, M. W., Pellegrini, E., Gueguen, M. M., Brion, F., ... Chung, B. C. (2009). A cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Genesis, 47(2), 67-73. https://doi.org/10.1002/dvg.20459

A cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. / Tong, Sok Keng; Mouriec, Karen; Kuo, Ming Wei; Pellegrini, Elisabeth; Gueguen, Marie Madeleine; Brion, François; Kah, Olivier; Chung, Bon Chu.

In: Genesis, Vol. 47, No. 2, 01.07.2009, p. 67-73.

Research output: Contribution to journalArticle

Tong, SK, Mouriec, K, Kuo, MW, Pellegrini, E, Gueguen, MM, Brion, F, Kah, O & Chung, BC 2009, 'A cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells', Genesis, vol. 47, no. 2, pp. 67-73. https://doi.org/10.1002/dvg.20459
Tong SK, Mouriec K, Kuo MW, Pellegrini E, Gueguen MM, Brion F et al. A cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Genesis. 2009 Jul 1;47(2):67-73. https://doi.org/10.1002/dvg.20459
Tong, Sok Keng ; Mouriec, Karen ; Kuo, Ming Wei ; Pellegrini, Elisabeth ; Gueguen, Marie Madeleine ; Brion, François ; Kah, Olivier ; Chung, Bon Chu. / A cyp19a1b-GFP (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. In: Genesis. 2009 ; Vol. 47, No. 2. pp. 67-73.
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