A decapeptide, growth stimulator identified from natural rubber serum powder for Bifidobacterium bifidum

Shin Ichi Etoh, Kayoko Asamura, Azumi Obu, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticlepeer-review

Abstract

Natural rubber serum powder, which is by-product obtained in the production of latex rubber, has a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254. The retained fraction obtained by ultrafiltration (molecular weight cutoff 1000) showed a growth-stimulating activity in a dose-dependent manner on B12 assay medium with ammonium sulfate. One of the growth stimulators was purified from the retained fraction by acetone precipitation, solid-phase extraction with a hydrophobic pretreatment column, and multi-stage reversed-phase HPLC. An increase of 53-fold in the specific activity, and a recovery of 1.3% were obtained. The amino acid composition and N-terminal sequence analyses of this growth stimulator provided the structure of Ala-Thr-Pro-Glu-Lys-Glu-Glu-Pro-Thr-Ala. The molecular mass was 1075 by MALDI-TOF MS analysis. These results showed that this growth stimulator was a decapeptide with the sequence shown above. This is the first report that clarified the structure of an active peptide for the growth of Bifidobacterium.

Original languageEnglish
Pages (from-to)171-181
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number1
Publication statusPublished - Nov 2000

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

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